蓝色鳞（Cyprinus carpio blue vat）的细胞遗传学分析

采用体内注射PHA和秋水仙素，肾细胞短期培养，常规空气干燥法制备蓝色鳞鲤染色体，对100个中期分裂相记数统计，确定蓝色鳞鲤（Cyprinus carpio blue var）的染色体数为2n=100。测得核型参数按Levan等的染色体划分标准得出：蓝色鳞鲤有15对中部着丝点染色体（m）；13对亚中部着丝点染色体（sin）；22对端部和亚端部着丝点染色体（st，t），其染色体总臂数（NF）为156，蓝色鳞鲤核型公式为2n=30m＋26sm＋44st，t。采用流式细胞分析仪测定了蓝色鳞鲤的DNA含量，与鸡血细胞标准对照相比为1．73±0．10，以鸡红细胞DNA含量2．3Pg．N^-1计，则蓝色鳞鲤的体细胞DNA含量为3．99Pg．N^-1。

Cytogenetic Analysis of Blue Common Carp Cyprinus carpio blue var

The diploid chromosome number, karyotype, and DNA content were studied in metaphase chromosome in kidney cells and blood of blue common carp Cyprinus carpio blue var injected with PHA and colchicin by airdrying technique. It was found that the diploid chromosome number was 2n=100. The karyotype was found to be composed of 30 m, 26sm and 44st＋t chromosomes, and NF=156. The diploid nucleus DNA content was measured from the somatic cell of the blue common carp using flow cytometer （Beckman cellLab Quant, made in USA） and the DNA content （2.3 pg· N^＋） in erythrocytes of chick （Gallus sp） as standard. The common carp had DNA content of3.99pg·N^-1, corresponding to the result measured chromosome number as ploidy.