Identification of two SCAR markers co-segregated with the dominant Ms and recessive ms alleles in onion (Allium cepa L.) |
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Authors: | Yan Yan Yang Yu Meng Huo Jun Miao Bing Jiang Liu Su Ping Kong Li Min Gao Chang Liu Zhen Bao Wang Yasuki Tahara Hidemi Kitano Xiong Wu |
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Institution: | 1. Vegetable Research Institute of Shandong Academy of Agricultural Sciences, Ji’nan, 250100, China 2. Key Laboratory for Biology of Greenhouse Vegetable of Shandong Province, Ji’nan, 250100, China 3. National Center for Vegetable Improvement (Shandong Branch), Ji’nan, 250100, China 4. College of Landscape and Horticulture, Qingdao Agricultural University, Qingdao, 266109, China 5. Technical Center of Nagoya University, Nagoya, 464-8601, Japan 6. Bioscience and Biotechnology Center, Nagoya University, Nagoya, 464-8601, Japan
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Abstract: | Cytoplasmic genetic male-sterility is used to produce hybrid onion (Allium cepa L.) seeds worldwide. In this paper, we present the results of research aimed toward identifying PCR-based markers linked to the Ms locus through amplified fragment length polymorphism (AFLP). After screening 512 AFLP primer combinations, only one AFLP fragment was identified as being flanking linked to the dominant Ms allele. Subsequently, the AFLP marker was converted into a sequence-characterized amplified region (SCAR) marker, designated as DNF-566, co-segregated with the dominant Ms allele in first backcross (BC1) segregated populations. Furthermore, we designed another molecular marker (RNS-357) co-segregated with the ms allele to identify different genotypes (i.e., MsMs, Msms, or msms). Both markers could be used for evaluating onion lines with different genetic backgrounds (including male-sterile lines, maintainer lines, male-fertile lines, and commercial based F1 hybrid cultivars). The results of this study indicate that maintainer plants could be directly selected by using these 2 SCAR markers in the onion breeding process, and this may contribute significantly toward breeding onion F1 hybrid cultivars. |
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