| 焦磷酸测序鉴别猪线粒体细胞色素b基因单倍型 |
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| 引用本文： | 蒋思文,Leif Andersson. 焦磷酸测序鉴别猪线粒体细胞色素b基因单倍型[J]. 中国兽医学报, 2002, 22(1): 44-46 |
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| 作者姓名： | 蒋思文 Leif Andersson |
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| 作者单位： | 华中农业大学，Department of Animal Breeding and Genetics Swedish University of Agricultural Science，Uppsala Biomedical Centre，Department of Animal Breeding and Genetics Swedish University of Agricultural Science，Uppsala Biomedical Centre 湖北武汉430070.Dep |
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| 基金项目： | 国家“973”重大基础研究项目 (G2 0 0 0 -0 1610 5 )~~ |
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| 摘 要： | 为了鉴别猪线粒体细胞色素 b(cytochrom e B,cyt b)基因单倍型 ,选择 9个不同品种共 4 15头猪作为试验材料 ,采用实时 DNA测序新方法焦磷酸测序 ,分析了 cyt b单核苷酸多态性 (single nucleotide polymorphism s,SNPs)。分析结果显示 3种不同单倍型 E、A1和 A2。除 1头临高猪属于单倍型 A2外 ,其他 6个中国地方品种猪均属于单倍型A1;瑞典长白猪和大白猪存在单倍型 E和 A1,而皮特兰猪存在单倍型 E和 A2。此外 ,基于焦磷酸测序 ,在通城猪中发现了 1个新的 SNP位点。
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| 关 键 词： | 线粒体DNA 细胞色素b基因 单倍型 焦磷酸测序 |
Haplotypes Detection of the Pig Mitochondrial Cytochrome B Gene by Pyrosequencing |
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| Elisabetta Giuffra,Leif Andersson. Haplotypes Detection of the Pig Mitochondrial Cytochrome B Gene by Pyrosequencing[J]. Chinese Journal of Veterinary Science, 2002, 22(1): 44-46 |
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| Authors: | Elisabetta Giuffra Leif Andersson |
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| Abstract: | To detect porcine mitochondrial cytochrome b (cyt b) gene haplotypes, pyrosequencing, a real time DNA sequence method, was used to analyze SNPs selected a total of 415 pigs from 9 different breeds. Pyrosequencing analysis of cyt b gene displayed 3 distinct haplotypes E, A1, A2, respectively. Except for only 1 Qingping pig, the other Chinese pigs from 6 unrelated breeds only present the haplotype A1. Swedish Large White and Landrace pigs present the haplotype E and A1 whereas Pietrain pigs present the haplotype E and A2. In addition, we found a new SNP in Tongcheng pigs based on pyrosequencing. |
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| Keywords: | mitochondrial DNA cytochrome b gene haplotype pyrosequencing |
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