萃取物化学成分分析

利用超临界CO2萃取紫丁香干花,提取物在分离釜Ⅰ(8 MPa,45℃)中进行第一级分离,再在分离釜Ⅱ(5 MPa、35℃)中进行第二级分离。通过GC-MS分析了两个分离釜中萃取物的化学成分并进行比较。从分离釜Ⅰ中所得萃取物中鉴定出21种成分,以长链烷烃为主(占57.14%),主要成分为二十九烷和2,6,10,14-四甲基十六烷;从分离釜Ⅱ所得萃取物中鉴定出40种成分,以含氧化合物为主,醇、酮、醛、酸、酯占67.5%,主要成分为十六烷酸和(Z,Z)-9,12-十八碳二烯酸。二级分离可使紫丁香干花超临界CO2萃取物得到一定程度分离,温度和压力是制约分离结果的关键因素。

Analysis of the Chemical Constituents by Supercritical CO2 Extraction from Dried Flower of Syringa oblata Lindl

Dried flowers of Syringa oblata Lindl.were extracted by supercritical CO2 and the extracts were separated in separators Ⅰ(8 MPa,45 ℃) and Ⅱ(5 MPa,35 ℃).The chemical compositions of the extract from the separators Ⅰ and Ⅱ were analysed by GC-MS.21 Different compounds in the obtained extract from the separatorⅠwere identified and were mainly long-chain alkane(57.14 %) with nonacosane and 2,6,10,14-tetramethyl hexadecane as major compounds.Forty compounds in the extract from the separator Ⅱ were identified and were mainly oxy-compounds with hexadecanoic acid and 9,12-octadecadienoic acid(Z,Z) as major components,such as alcohols,ketones,aldehydes,acids and esters accounting for 67.5 %.The supercritical CO2 extracts can be separated in certain extent by the two-stage separation.Temperature and pressure are the key factors for the separation results.