灯盏细辛离体叶片再生植株的研究

以灯盏细辛（Erigeron breiscapus）叶片为外植体，研究不同培养基配方对灯盏细辛离体培养过程中愈伤组织、不定芽形成，壮苗培养和离体植株生根等方面的影响。研究结果表明，低浓度的细胞分裂素与生长素配比可诱导灯盏细辛愈伤组织产生，其中MS＋BA1．00mg／L＋NAA0．50mg／L培养基配方，愈伤组织诱导率达到99．22％；不定芽诱导需要较高浓度的细胞分裂素，适宜培养基MS＋KT4．00mg／L＋IBA0．50mg／L的不定芽诱导率为38．51％，诱导系数为0．49；MS＋BA0．50mg／L＋NAA0．50mg／L＋水解酪蛋白1000．00mg／L＋PVP1000．00mg／L＋GA0．50mg／L培养基可促进不定芽分化生长，形成离体植株；离体植株生根需要高浓度生长素，适宜培养基为1／2MS＋BA0．50mg／L＋NAA3．00mg／L＋IBA3．00mg／L＋活性炭0．30％。

Plant Regeneration of Erigeron breviscapus Through Culture of Leaves in Vitro

Effect of different mediums on callus induction, adventitious bud induction, bud culture and root formation of Erigeron breiscapus was studied, using leaves as explants. The callus was induced from the explant of leave with mediums containing low concentration of cytokinin and auxins. The highest induction rate of callus reached 99.22% on medium： MS ＋ BA 1.00mg/L ＋ NAA 0.50 mg/L. High con-centration of cytokinin was necessary to adventitious bud induction. The induction rate of adventitious bud was 38.51% and number of shoots per explant was 0.49 on medium： MS ＋ KT 4.00mg/L ＋ IBA 0.50mg/L. The bud easily developed into plantlet cultured on medium： MS ＋ BA 0.50mg/L ＋ NAA 0.50 mg/L＋casein hydrolysate 1000.00 mg/L ＋PVP 1000.00 mg/L＋ GA 0.50mg/L. Root formation need medium containing high concentration of auxins. The optimal medium for root formation was 1/2MS ＋ BA 0.50mg/L ＋ NAA 3.00 mg/L ＋ IBA 3.00 mg/L ＋ activated charcole 0.30%.