| 野生型拟南芥叶片愈伤组织诱导的研究 |
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| 引用本文： | 林呐,余韩开宗,肖丽娜,李春丽.野生型拟南芥叶片愈伤组织诱导的研究[J].农业科学与技术,2011(1):82-84,107. |
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| 作者姓名： | 林呐 余韩开宗 肖丽娜 李春丽 |
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| 作者单位： | 西南大学农学与生物科技学院重庆市油菜工程技术研究中心 |
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| 基金项目： | Supported by Basic Scientific Research Special Found of Central College(100030-2120131106)~~ |
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| 摘 要： | 目的]研究野生型拟南芥叶片愈伤组织的诱导。方法]以野生型拟南芥叶片为外植体,通过将其切段后接种在添加有不同浓度6-BA和NAA的MS培养基上,研究了拟南芥愈伤组织的诱导和再生植株。结果]6-BA对于愈伤组织诱导不可缺少,但其浓度太高易产生玻璃化;NAA单独使用利于生根,配合6-BA使用利于分化芽;获得最适愈伤组织诱导培养基为MS＋0.50mg/L6-BA＋0.10mg/LNAA,愈伤组织诱导率可达100%。结论]为进一步开展拟南芥的遗传转化或细胞培养奠定了基础。
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| 关 键 词： | 拟南芥 叶片 愈伤组织 |
Study on the Callus Induction of Wild Arabidopsis thaliana Leaves |
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| LIN Na,YUHAN Kai-zong,XIAO Li-na,LI Chun-li.Study on the Callus Induction of Wild Arabidopsis thaliana Leaves[J].Agricultural Science ＆ Technology,2011(1):82-84,107. |
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| Authors: | LIN Na YUHAN Kai-zong XIAO Li-na LI Chun-li |
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| Institution: | College of Agronomy and Biotechnology,Southwest University,Research Center of Rape Engineering and Technology in Chongqing,Chongqing 400715 |
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| Abstract: | Objective] The callus induction of wild Arabidopsis thaliana leaves was studied.Method] As explants,leaf segments of wild A.thaliana were inoculated in MS medium including 6-BA and NAA with different concentration,and studying the callus induction and regenerated plant of A.thaliana.Result]6-BA was necessary for callus induction,but higher concentration resulted into vitrification easily;single use of NAA was beneficial to rooting,and bud differentiation was easy in the medium with NAA and 6-BA;the optimum medium of callus induction was MS +0.5 mg/L 6-BA +0.10 mg/L NAA,with the callus induction frequency of 100%.Conclusion]Our study could lay a foundation for the genetic transformation and cell culture of A.thaliana. |
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| Keywords: | Arabidopsis thaliana Leaf Callus |
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