| 重组的马铃薯X病毒载体诱导烟草rbcS基因沉默 |
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| 引用本文： | 周晓馥,朱筱娟,刘文波,王仁厚,王兴智. 重组的马铃薯X病毒载体诱导烟草rbcS基因沉默[J]. 分子植物育种, 2004, 2(2): 197-201 |
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| 作者姓名： | 周晓馥 朱筱娟 刘文波 王仁厚 王兴智 |
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| 作者单位： | 东北师范大学遗传与细胞研究所,长春,130024;吉林师范大学基因工程研究所,四平,136000;东北师范大学遗传与细胞研究所,长春,130024;中国科学院遗传与发育生物学研究所,北京,100101 |
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| 摘 要： | 用重组马铃薯病毒X(potato virus X，PVX)载体侵染烟草植株(Nicotiana benthamiana)，该载体带有与RubisCO小亚基(ribulose bisphosphate carboxylase small subunit，rbcS)基因同源的500个核苷酸的cDNA片段。2～3周后，烟草植株出现退绿的rbcS基因沉默的明显症状。利用烟草叶片总RNA，进行Northern杂交检测内源rbcS的表达和病毒复制的情况表明，受病毒侵染的植株内源rbcS基因的表达受到了明显的抑制，而病毒的复制并没有受到影响，重组的病毒载体上与内源基因同源的核苷酸序列诱导了内源rbcS基因的沉默。
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| 关 键 词： | Northern杂交 1 5-二磷酸核酮糖羧化酶/加氧酶小亚基 病毒诱导基因沉默 |
Potato Virus X Vector Induced Silencing of rbcS Gene in Nicotiana benthamiana |
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| Zhou Xiaofu , Zhu Xiaojuan Liu Wenbo Wang Renhou Wang Xingzhi Institute of Genetics and Cytology,Northeast Normal University,Changchun. Potato Virus X Vector Induced Silencing of rbcS Gene in Nicotiana benthamiana[J]. Molecular Plant Breeding, 2004, 2(2): 197-201 |
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| Authors: | Zhou Xiaofu Zhu Xiaojuan Liu Wenbo Wang Renhou Wang Xingzhi Institute of Genetics Cytology Northeast Normal University Changchun |
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| Affiliation: | Zhou Xiaofu 1,2 Zhu Xiaojuan 1 Liu Wenbo 3 Wang Renhou 1 Wang Xingzhi 1* 1 Institute of Genetics and Cytology,Northeast Normal University,Changchun,130024 2 Institute of Gene Technology,Jilin Normal University,Siping,136000 3 Institute of Genetics Developmental Biology,Chinese Academy of Sciences,Beijing,100101 |
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| Abstract: | |
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| Keywords: | Northern hybridization Ribulose bisphosphate carboxylase/oxydase small subunit(rbcS) Virus-induced gene silencing (VIGS) |
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