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美凤蝶非滞育蛹·滞育蛹与滞育蛹羽化成虫核酸含量比较
引用本文:易传辉,陈晓鸣,史军义,周成理.美凤蝶非滞育蛹·滞育蛹与滞育蛹羽化成虫核酸含量比较[J].农业科学与技术,2009,10(3):101-103,107.
作者姓名:易传辉  陈晓鸣  史军义  周成理
作者单位:易传辉,YI Chuan-hui(中国林业科学研究院资源昆虫研究所,国家林业局资源昆虫培育与利用重点实验室,云南昆明,650224;云南林业职业技术学院,云南昆明,650224);陈晓鸣,史军义,周成理,CHEN Xiao-ming,SHI Jun-yi,ZHOU Cheng-li(中国林业科学研究院资源昆虫研究所,国家林业局资源昆虫培育与利用重点实验室,云南昆明,650224) 
基金项目:国家林业局引进国际先进林业科学技术计划项目 
摘    要:1材料与方法 1.1材料美凤蝶为四川省峨眉山市人工养殖的同一批滞育蛹,化蛹时间相近(9月中旬左右)。

关 键 词:美凤蝶  非滞育蛹  滞育蛹  羽化成虫  核酸含量  人工养殖

Comparison of Nucleic Acid Content among Non-diapause Pupae, Diapause Pupae and Eclosion-adult from Diapause Pupae of Papilio memnon
YI Chuan-hui,CHEN Xiao-ming,SHI Jun-yi,ZHOU Cheng-li.Comparison of Nucleic Acid Content among Non-diapause Pupae, Diapause Pupae and Eclosion-adult from Diapause Pupae of Papilio memnon[J].Agricultural Science & Technology,2009,10(3):101-103,107.
Authors:YI Chuan-hui  CHEN Xiao-ming  SHI Jun-yi  ZHOU Cheng-li
Institution:1. Research Institute of Resources Insects of Chinese Academy of Forestry, Key laboratory of Resource Insect Breeding and Utilization, State Forestry Administration, Kunming 650224; 2. Yunnan Forestry Technological College, Kunming 650224)
Abstract:Objective] The aim of this study was to provide basis for deeply understanding the diapause mechanism of Papilio memnon L. Method] RNA and DNA content of non-diapause pupae, diapause pupae and eclosion-adult from diapause pupae at different development stages were detected by the colorimetry. Result] RNA content of non-diapause pupae was 4.614 0-7.946 3 μg/mg, while diapause pupae was 4.326 0-5.885 3 μg/mg and eclosion-adult from diapause pupae was 20.779 3 μg/mg at initial stage. DNA content of non-diapause pupae was 0.448 7-0.535 0 μg/mg, while diapause pupae was 0.452 0-0.828 3 μg/mg and eclosion-adult from diapause pupae was 1.727 0 μg/mg at initial stage. Conclusion] The nucleic acid content and change is related to the development stage.
Keywords:Papilio memnon  Nucleic acid content  Diapause  Pupae  A dult
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