| 口蹄疫A型病毒AF/72株标准细胞毒株的研制 |
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| 引用本文： | 杜进鑫,王永录,张永光,方玉珍,蒋守田,吕建亮,潘丽,刘力宽. 口蹄疫A型病毒AF/72株标准细胞毒株的研制[J]. 华北农学报, 2009, 24(3) |
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| 作者姓名： | 杜进鑫 王永录 张永光 方玉珍 蒋守田 吕建亮 潘丽 刘力宽 |
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| 作者单位： | 中国农业科学院兰州兽医研究所,家畜疫病病原生物学国家重点实验室,农业部畜禽病毒学重点开放实验室,甘肃兰州,730046;甘肃农业大学动物医学院,甘肃兰州,730070;中国农业科学院兰州兽医研究所,家畜疫病病原生物学国家重点实验室,农业部畜禽病毒学重点开放实验室,甘肃兰州,730046 |
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| 摘 要： | 用口蹄疫A型病毒AF/72株的第3代乳鼠组织毒,通过乳鼠继续适应3代后,转适BHK-21细胞,获得该毒株的细胞毒AF/72/MF6/BF12,经测定其TCID50为10-8.0/mL;经RT-PCR获得其VPI基因序列,并与GenBank中的其他6株口蹄疫A型病毒株比对,同源性均大于85%;经无菌检验和外源病毒检验,纯净性达到兽用生物制品标准要求;经间接夹心ELISA测定,OD值均大于0.2,且该毒仅能被口蹄疫A型标准血清中和,具有型特异性;经紫外分光光度法测定其146S含量,均值为189 ng/mL,远大于22 ng/mL的国际标准.综合纯净性检验结果、特异性检验结果和146S含量,确定AF/72/MF6/BF12为口蹄疫A型病毒株AF/72的标准细胞毒.
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| 关 键 词： | 口蹄疫A型病毒 FMD 毒株 |
Study of Reference Cellular Strain of AF/72 of FMD Type A Virus |
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| DU Jin-xin,WANG Yong-lu,ZHANG Yong-guang,FANG Yu-zhen,JIANG Shou-tian,LU Jian-liang,PAN Li,LIU Li-kuan. Study of Reference Cellular Strain of AF/72 of FMD Type A Virus[J]. Acta Agriculturae Boreali-Sinica, 2009, 24(3) |
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| Authors: | DU Jin-xin WANG Yong-lu ZHANG Yong-guang FANG Yu-zhen JIANG Shou-tian LU Jian-liang PAN Li LIU Li-kuan |
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| Affiliation: | 1.Key Laboratory of Animal Virology of Agriculture;State Key Laboratory of Veterinary Etiological Biology;Lanzhou Veterinary Research Institute;Chinese Academy of Agriculture Sciences;Lanzhou 730046;China;2.College of Veterinary Medicine;Gansu Agricutural University;Gansu 730070;China |
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| Abstract: | Get the foot-and-mouth virus AF/72/MF6/BF12 by rat rejuvenation and BHK-21 cells adaption using FMD virus AF/72/MF3 the use of Karber method to measure AF/72/MF6/BF12 TCID50.its value was 8.0;the main antigen VP1 gene sequence was got by RT-PCR with 6 type A strains of FMDV in GenBank,homology was higher than 85%.By the sterile examination,other exogenous virus test,its purity of AF/72/MF6/BF12 conform to Biological Products Standards for Animal Use;Indirect Sandwich ELISA OD Values greater than 0.2,This vi... |
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| Keywords: | FMD |
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