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辽宁绒山羊全基因组外显子测序研究
引用本文:郭 丹 张兴会 韩 迪. 辽宁绒山羊全基因组外显子测序研究[J]. 现代畜牧兽医, 2014, 0(3): 21-24
作者姓名:郭 丹 张兴会 韩 迪
作者单位:辽宁省畜牧科学研究院,辽宁 辽阳111000
基金项目:辽宁省科学事、№公益研究基金(2012005005)
摘    要:本研究应用全外显子捕获测序分析技术构建绒山羊的cDNA文库,研发绒山羊外显子捕获试剂盒进行测序研究。结果共找出9974个突变或SNP位点,其中2组羊中共享的SNP总共4254个,季节性长绒型的群体中独有的SNP有2713个,常年长绒型的群体中独有的SNP有3007个,为常年长绒辽宁绒山羊主效调控基因的QTL定位提供理论依据。

关 键 词:外显子  辽宁绒山羊  测序

Study on the whole exons genome sequencing of Liaoning cashmere goats
Guo Dan,Zhang Xinghui,Han Di. Study on the whole exons genome sequencing of Liaoning cashmere goats[J]. Modern JOurnal of Animal Husbandry and Veterinary Medicine, 2014, 0(3): 21-24
Authors:Guo Dan  Zhang Xinghui  Han Di
Affiliation:(Animal Husbandry Institute of Liaoning Province, Liaoning Liaoyang 111000)
Abstract:In this study, whole exons sequencing captured technology was used to construct a cDNA library of cashmere goat, and in order to explore a cashmere goat research exons capture kit for sequencing studies. Finally, 9 974 SNPs were found, which included 4 254 shared SNPs of the two type goat, 2 713 unique SNPs of the seasonal-growth cashmere type and 3 007 SNPs unique SNPs of the laster-growth cashmere type. The study provides a theoretical basis for the QTL of last-growth cashmere gene.
Keywords:Exons genome  Liaoning cashmere goats  Sequencing
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