基于地理种源分组和表型数据构建尾叶桉核心种质

建立尾叶桉的核心种质可以快速、准确地从丰富的尾叶桉种质资源中鉴定出育种上迫切需要的优异基因源。鉴于此,以尾叶桉家系表型性状的均值、方差、极差、变异系数和平均多样性指数5个参数作为评价指标,按照不同取样比例,筛选尾叶桉初级核心种质;然后按照地理种源分组,利用均值差异百分率、变异系数变化率、极差符合率、方差差异百分率4个参数对不同取样比例下的核心种质进行比较分析,以最小距离逐步取样法构建尾叶桉二级核心种质,共得188份,占资源总量的10%,且与控制不同性状表型相关的遗传特性在核心种质中也得到了较好的保持。所构建的尾叶桉二级核心种质可以作为整体资源的代表性样本。

Establishment of Eucalyptus urophylla Core Collection Based on Geographical Distribution and Phenotypic Data

Establishing core collection of Eucalyptus urophylla can identify quickly and accurately the superior gene sources from its rich and diversified germplasm resources that is urgently requested by breeding practice. The primary core collection of E. urophylla was selected by different sampling proportion according to 5 parameter values, i. e. , the mean value, variance, range, coefficient of variation and average diversity index values of the E. urophylla phenotypic properties of the genealogies. The primary core collection was grouped by the geographical provenances, then compared and analyzed by different sampling proportion according to the 4 parameters including MD, VR, CR, VD to build the secondary core collection of E. urophyUa by means of LDSS method. As the result, totally 188 pieces of secondary core collection were screened out which accounted for 10% of the whole resources. It was tested that the genetic characteristics controlling different character phenotypic correlation were also well remained in the secondary core collection. The constructed secondary core collection resources of E. urophylla might be treated as the representative samples, which would play a very important role in utilizing the E. urophylla resources.