| Ri质粒转化牛膝及其发状根培养 |
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| 引用本文： | 牛膝谭题组. Ri质粒转化牛膝及其发状根培养[J]. 郑州牧业工程高等专科学校学报, 1997, 0(4) |
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| 作者姓名： | 牛膝谭题组 |
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| 作者单位： | 郑州牧专 |
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| 基金项目： | 河南省自然科学基金资助项目 |
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| 摘 要： | 用发根农杆菌(Agrobacterium rhizogenes)的Ri质粒转化药用植物牛膝(Achyranthes bidentataBl.),诱导出了抗卡那霉素的发状根。将诱导的发状根进行固体平板培养和液体悬浮培养。在附加Km30mg/L的MS。和1/2MS。培养基上,发状根生长良好,而未经转化的对照组则死亡,表现出生长激素自主性。在附加外源激素IBA0.1mg/L和NAA0.1mg/L时。能促进发状根的成活、侧根的形成以及提高增殖倍数。表明适量的外源激素能促进发状根的生长。
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| 关 键 词： | 牛膝 发根农杆菌 转化 发状根培养 |
TRANSFORMATION OF Achyranthes bidentatoBL. By Ri PLASMID AND THE HAIRY ROOT CULTURE |
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| The Research Group. TRANSFORMATION OF Achyranthes bidentatoBL. By Ri PLASMID AND THE HAIRY ROOT CULTURE[J]. Journal of Zhengzhou College of Animal Husbandry Engineering, 1997, 0(4) |
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| Authors: | The Research Group |
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| Affiliation: | Zhengzhou College of Animal Husbandry Engineering |
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| Abstract: | Achyranthes bidentata BL, a famous medica plant, was transferred by Ri plasmid with Agrobac-terium rhizogenes, and hairy root with kanamycin resistance was induced successfully. The induced toots were cultured in solid media and liquid media. The excised hairy roots grew very well on MS and 1/2 MS mediawhich both contained Km30mg/L, whereas the control group can not. These results showed that the hairy root has the ability of auxin synthesis by itself. Media containing IBAO. 1mg/L or NAA0. 1mg/L can increase the hairy root survival rate, lateral root formation and cultural production, which demonstrated that proper foreign auxin can promote the growth of hairy root. |
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| Keywords: | Achyranthes bidentata Agrobacterium rhizogenes transformation hairy root culture |
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