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外源纤维改良基因对棉花纤维品质的影响研究
引用本文:张震林,周宝良,刘正銮,陈松,张香桂.外源纤维改良基因对棉花纤维品质的影响研究[J].华中农业大学学报,2004,23(2):192-196.
作者姓名:张震林  周宝良  刘正銮  陈松  张香桂
作者单位:1. 江苏省农业科学院经济作物研究所,南京,210014
2. 江苏省太仓棉花原种场,太仓,215411
基金项目:江苏省农业高技术项目(BG2002307)资助
摘    要:将由棉纤维细胞特异表达启动子驱动的外源纤维改良基因(兔角蛋白基因),通过花粉管通道转基因法,导人陆地棉品种(系)苏棉16号、泗棉167、渝棉1号和H1。检测结果表明,转兔角蛋白基因苏棉16号和泗棉167的纤维比强度分别比相应对照增加了23.2%和29.6%,马克隆值下降了13.3%和17.0%,纤维长度与对照接近。结果还表明,外源基因对高品质棉花品种(系)渝棉1号和H1纤维品质的影响不明显,只是衣分有所增加。

关 键 词:棉花  纤维品质  纤维改良基因  转基因  花粉管通道

Effects of a Foreign fiber-Improving Gene on Fiber Properties ofCotton (Gossypium hirsutum L)
Zhang Zhenlin Zhou Baoliang Liu Zhengluan Chen Song Zhang Xianggui.Effects of a Foreign fiber-Improving Gene on Fiber Properties ofCotton (Gossypium hirsutum L)[J].Journal of Huazhong Agricultural University,2004,23(2):192-196.
Authors:Zhang Zhenlin Zhou Baoliang Liu Zhengluan Chen Song Zhang Xianggui
Abstract:The foreign fiber-improving gene, a rabbit keratin gene driven by a fiber-specific E6 promoter, was introduced into Cotton (Gossypium hirsutum L. ) plants of the cultivar Sumianl6, Simi-an167, Yumianl, and H1, by means of pollen tube-mediated transformation. The values of 2. 5% span length(SL), strength, and micronaire of the fiber properties and lint percentage were determined to e-valuate the fiber quality of the rabbit keratin-transgenic cotton. The results indicated that the fiber strength of keratin-transgenic Sumian16 and Simian167 were increased, compared with its own control, by 23. 2% and 29. 6% respectively, and micronaire values decreased by 13. 3% and 17. 0% respectively. The results also revealed no effects of the foreign fiber-improving gene on the fiber properties of the high-quality cotton cultivars Yumianl and HI, but some increase only in lint percentage.
Keywords:cotton(Gossypium hirsutum L  )  transgene  fiber-improving gene  fiber quality
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