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| 矮牵牛花药培养的研究 | |
| 引用本文： | 庞海峰,王平,姜策. 矮牵牛花药培养的研究[J]. 北方园艺, 2007, 0(5): 196-197 |
| 作者姓名： | 庞海峰 王平 姜策 |
| 作者单位： | 1. 沈阳农业大学园艺学院,110161 2. 辽宁省农业科学院,沈阳,110161 3. 辽宁省植物保护站,沈阳,110034 |
| 摘 要： | 研究了不同材料消毒方式、不同种类和浓度的激素对矮牵牛花药培养的影响.结果表明:1mol/L HCL 1min 75%酒精30s 0.1%升汞8min的消毒方式效果最佳;Nitsch 2,4-D0.2 NAA0.5 BA0.2的愈伤组织诱导率最高;分化培养基中只有MS NAA 0.1 BA 2.0形成了不定芽,且分化率较低,为23.8%,针对这一问题需要做更为深入的研究. |
| 关 键 词： | 矮牵牛 花药培养 愈伤组织 |
| 文章编号： | 1001-0009(2007)05-0196-02 |
| 收稿时间： | 2006-12-13 |
| 修稿时间： | 2006-12-13 |
Research on Anther Culture of Petunia |
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| PANG Hai-feng,WANG Ping,JIANG Ce. Research on Anther Culture of Petunia[J]. Northern Horticulture, 2007, 0(5): 196-197 | |
| Authors: | PANG Hai-feng WANG Ping JIANG Ce |
| Affiliation: | 1. Horticulture Academy of Shenyang Agricultural University,110161; 2. Liaonlng Academy of Agricultural Sciences,Shenyang, 110161;3. Liaoning Plant Protection Station,Shenyang 110034 |
| Abstract: | This paper evaluated the effects of different ways of explant sterilization,different kinds and different concentrations of hormone on anther culture of Petunia.The results were as follows:the most optimal method of sterilization was 1mol/L HCL1min 75%alcohol 30sec 0.1%HgCl2 8min;Nitsch 2,4-D 0.2 NAA0.5 BA0.2 was the best induced medium;among all division mediums,only MS NAA 0.1 BA 2.0 formed shoots,but the rate of division was low,we still need further evaluation. |
| Keywords: | Petunia Anther culture Callus |
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