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| 水稻光敏色素相互作用因子基因OsPIL11的表达模式及其在光信号传导中作用的初步分析 | |
| 引用本文： | 梁卫红,李莉,彭威风,刘婧,杨慧,谢先芝. 水稻光敏色素相互作用因子基因OsPIL11的表达模式及其在光信号传导中作用的初步分析[J]. 中国水稻科学, 2012, 26(3): 255-260. DOI: 10.3969/j.issn.1001 7216.2012.03.001 |
| 作者姓名： | 梁卫红 李莉 彭威风 刘婧 杨慧 谢先芝 |
| 作者单位： | 1. 河南师范大学生命科学学院,河南新乡,453007 2. 河南师范大学生命科学学院,河南新乡453007;山东省农业科学院高新技术研究中心,山东济南250100 3. 山东省农业科学院高新技术研究中心,山东济南,250100 4. 山东省农业科学院高新技术研究中心,山东济南250100;农业部黄淮海作物遗传改良与生物技术重点开放实验室,山东济南250100 |
| 基金项目： | 国家自然科学基金资助项目(30971744,31171182);河南省科技创新杰出青年计划资助项目(104100510012) |
| 摘 要： | 分析了OsPIL11基因(6个潜在的PIF转录因子之一)的表达模式,结果表明OsPIL11在水稻中的表达具有器官特异性,并且受叶片发育、脱落酸、茉莉酮酸和水杨酸调控。为了进一步分析OsPIL11在植物光信号传导中的作用,构建了OsPIL11基因的过量表达载体,并将其转化至烟草。通过比较转基因和野生型烟草植株幼苗在红光和远红光下的光形态建成特征,发现红光下生长的OsPIL11转基因烟草下胚轴较短、真叶和子叶较大;然而在远红光下,转基因和野生型烟草植株的表型没有差异。这些结果表明,OsPIL11参与红光诱导的幼苗去黄化反应,但是不参与远红光诱导的幼苗去黄化反应。 |
| 关 键 词： | 水稻 光敏色素相互作用因子 转基因烟草 光信号传导 |
| 收稿时间： | 2011-08-29; |
Expression Patterns of OsPIL11,a Phytochrome-interacting Factor in Rice,and Preliminary Analysis of Its Roles in Light Signal Transduction |
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| LIANG Wei-hong , LI Li , PENG Wei-feng , LIU Jing , YANG Hui , XIE Xian-zhi. Expression Patterns of OsPIL11,a Phytochrome-interacting Factor in Rice,and Preliminary Analysis of Its Roles in Light Signal Transduction[J]. Chinese Journal of Rice Science, 2012, 26(3): 255-260. DOI: 10.3969/j.issn.1001 7216.2012.03.001 | |
| Authors: | LIANG Wei-hong LI Li PENG Wei-feng LIU Jing YANG Hui XIE Xian-zhi |
| Affiliation: | 2,3(1College of Life Science,Henan Normal University,Xinxiang 453007,China;2High-Tech Research Centre,Shandong Academy of Agricultural Sciences,Jinan 250100,China;3Key Laboratory of Crop Genetic Improvement and Biotechnology,Huanghuaihai,Ministry of Agriculture,Jinan 250100,China) |
| Abstract: | The expression patterns of OsPIL11,one of six putative phytochrome-interacting factors(PIFs),were analyzed,revealing that expression of OsPIL11was organ-specific and regulated by leaf development,ABA,JA and SA.To further explore the role of OsPIL11in plant light signal transduction,plant expression vector of OsPIL11was constructed and introduced into tobacco(Nicotiana tabacum).In continuous red light(R),OsPIL11-overexpressed transgenic lines exhibited shorter hypocotyls and larger cotyledons and leaves compared to wild-type seedlings.While in continuous far-red light(FR),either transgenic or wild-type seedlings showed similar phenotypes.These results indicated that OsPIL11was involved in R-induced de-etiolation,but not in FR-induced de-etiolation in transgenic tobacco. |
| Keywords: | rice phytochrome-interacting factor transgenic tobacco light signal transduction |
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