| 海南槟榔黄化病病原物的分子鉴定 |
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| 引用本文： | 车海彦,吴翠婷,符瑞益,温衍生,叶莎冰,罗大全.海南槟榔黄化病病原物的分子鉴定[J].热带作物学报,2010,31(1):83-87. |
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| 作者姓名： | 车海彦 吴翠婷 符瑞益 温衍生 叶莎冰 罗大全 |
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| 作者单位： | 1. 中国热带农业科学院环境与植物保护研究所,海南省热带农业有害生物检测监控重点实验室,海南儋州,571737
2. 海南大学环境与植物保护学院,海南儋州,571737蜒 |
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| 基金项目： | 国家自然科学基金资助项目，中央科研院所基本科研业务费专项，国家支撑计划课题 |
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| 摘 要： | 槟榔黄化病是槟榔上的一种重要病害,如何快速检测该病原菌是防治该病的重要基础。利用植原体16SrDNA通用引物对海南感染黄化病的槟榔花苞总DNA进行巢式PCR扩增,获得约1.2kb的特异片段,并对扩增产物进行核苷酸序列测定。通过BLAST程序比较、系统进化树构建及iPhyClassifier分析表明,引起海南槟榔黄化病病原植原体属于翠菊黄化植原体组(16SrⅠ组),且为该组中一个新的亚组,即G亚组,现将其暂命名槟榔黄化植原体(Arecanut yellow leaf phytoplasma,AYL)。
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| 关 键 词： | 槟榔黄化病 植原体 分子鉴定 |
Molecular Identification of Pathogens from Arecanut Yellow Leaf Disease in Hainan |
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| Che Haiyan,Wu Cuiting,Fu Ruiyi,Wen Yansheng,Ye Shabing and Luo Daquan.Molecular Identification of Pathogens from Arecanut Yellow Leaf Disease in Hainan[J].Chinese Journal of Tropical Crops,2010,31(1):83-87. |
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| Authors: | Che Haiyan Wu Cuiting Fu Ruiyi Wen Yansheng Ye Shabing and Luo Daquan |
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| Institution: | Che Haiyan1,2,Wu Cuiting3,Fu Ruiyi2,Wen Yansheng1,Luo Daquan1,2 1 Environment , Plant Protection Institute,CATAS,Danzhou,Hainan 571737,Hainan Key Laboratory for Detection , Monitoring of Tropical Agricultural Pests,2 College of Environment , Plant Protection,Hainan University |
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| Abstract: | Arecanut yellow leaf disease was an important disease infecting areca nut palm(Areca catechu L). A PCR product(about 1.2 kb) was amplified in nested-PCR assay with phytoplasma universal 16S rRNA gene primers, and the total DNA sample extracted from diseased areca nut inflorescence was used as the template. The amplified fragments were sequenced. Sequence comparison in the Blast program of GenBank, construction of phylogenetic tree and iPhyClassifier analysis indicated that the phytoplasma causing areca nut ... |
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| Keywords: | 16SrDNA areca nut yellow leaf disease phytoplasma 16S rDNA molecular identification |
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