新城疫植物油乳剂苗的研究 |
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引用本文: | 谢芝勋,刘加波,庞耀珊.新城疫植物油乳剂苗的研究[J].中国预防兽医学报,2000,22(4):262-266. |
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作者姓名: | 谢芝勋 刘加波 庞耀珊 |
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作者单位: | |
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摘 要: | 应用植物油代替矿物油制备ND油苗,植物油苗时相中含2.5%或5%蜂蜡(BW,乳化剂),油相和水相比例为4:1,植物油苗免疫来航易感鸡,并采血检测清HI抗体效价,全部5%BW植物油苗的HI抗体和对照矿物苗H抗体无明显差异(P〉0.05),而大多数2.5%BW植物油苗(2.5%BW花生油苗除外)及2.5%直溶BW植物油苗,大多数5%直溶BW植物疫苗95%直溶BW玉米油苗除外)的HI抗体都明显低于(P〈
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关 键 词: | 鸡新城疫 植物油乳剂苗 蜂蜡 疫苗 |
修稿时间: | 2000-01-11 |
Newcastle Disease Oil-emulsion Vaccines Prepared with Vegetable Oil |
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Abstract: | Vegetable oils were used as replacements for min
eral oil in ND oil-emulsion vaccines.2.5 percent or 5 percent of beewax(BW) of t he oil
phase that was used to prepare water-in-oil emulsion vaccines that contai ned a final
concentration of 20% aqueous antigen.White leghorn chickens were vaccina t ed and bled for
their serlogic HI titers testing.The HI titers of all vegetable oil vaccines with 5% BW
were not significantly different from those of control m ineral oil vaccine.but the HI
titers of most vegetable oil vaccines with 2.5%BW( except peanut oil vaccine with 2.5%
BW)were significantly lower than the control mineral oil vaccine.The HI titers of
vegetable oil vaccines with 2.5% and 5% di rect-melted BW (except the corn oil vaccine
with 5% direct-melted BW)were signif icantly lower than control mineral oil vaccine.Tissue
reactions associated with vegetable oil vaccines were less severe than those associated
with mineral oil v accine.These findings indicate that any of the several vegetable oil
may be more suitable than mineral oil for preparation of poultry oil-emulsion vaccines. |
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