Characterization of monoclonal antibodies directed against the canine distemper virus nucleocapsid protein |
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| Authors: | Masuda Munemitsu Sato Hiroki Kamata Hiroshi Katsuo Tomoe Takenaka Akiko Miura Ryuichi Yoneda Misako Tsukiyama-Kohara Kyoko Mizumoto Kiyohisa Kai Chieko |
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| Institution: | Department of Biochemistry, School of Pharmaceutical Sciences, Kitasato University, Shirokane, Minato-ku, Tokyo 108-8641, Japan. |
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| Abstract: | We have established four monoclonal antibodies (MAbs) against the nucleocapsid protein (NP) of canine distemper virus (CDV). A competitive binding assay has revealed that the MAbs are directed against two antigenic domains. An immunofluorescence assay using a series of deletion clones of the NP and an immunoprecipitation assay using the NP have revealed that two of the MAbs recognize the C-terminal region of the NP while the other two recognize the tertiary structure of the N-terminal domain. These MAbs reacted with all eight strains of CDV used in this study, but showed different reactivities against measles virus and rinderpest virus. |
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