冬凌草根际土壤微生物抗肿瘤活性菌株筛选

目的]为开发利用冬凌草根际土壤微生物资源。方法]使用K562细胞的SRB法筛选模型,对从贵州省施秉县采集的冬凌草根际土壤样品中分离的207株微生物(放线菌112株、真菌95株)进行了抗肿瘤活性筛选。结果]得到活性菌株共27株,阳性率为13.0%,其中真菌13株,放线菌14株。结论]冬凌草根际土壤微生物是寻找抗肿瘤活性代谢产物的良好资源。

Screening of Antitumor Strains from Rihizosphere Soil Microorganisms from Rabdosia rubescens

Objective] The research aimed to explore and utilize rihizosphere soil microorganism resources.Method] 207 strains of rihizosphere soil microorganisms,including 112 actinomycetes and 95 fungi,were isolated from Rabdosia rubescens in Shibing,Guizhou Province.And their fermented products were screened with K562 cells by SRB method.Result] 27 strains showed cell proliferation inhibitory activity on K562 cells,which possessed 13.0% of total 207 strains tested.In the active strains,there were 14 actinomycetes and 13 fungi, respectively.Conclusion] Rihizosphere soil microorganisms from Rabdosia rubescens were good resources for seeking antitumor metabolites.