中华绒螯蟹肝胰腺病症的病理分析

采用常规石蜡切片技术,对患肝胰腺病症的中华绒螯蟹不同组织进行病理学观察,同时测定肝胰腺中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(G-PX)3种抗氧化酶活性以及丙二醛(MDA)含量.结果显示,肝胰腺和鳃为主要病变组织,在病变最严重的蟹中,这两个器官的基本组织结构均完全崩解,呈一片无结构的物质,其他组织如心、肌肉也有不同程度的病变,肠中未发现有明显病变情况的发生；肝胰腺中3种抗氧化酶的活性以及MDA含量在不同程度病变的河蟹中都有较大程度的变化,其中肝胰腺组织轻微病变的河蟹肝胰腺中CAT活性呈现不显著变化,但随着病情的加重,3种酶活性及MDA含量均呈现显著差异.

Histopatholgical study on hepatopancreas symptoms of Eriocheir sinensis

Observations on the lesions in tissues and cells of Eriocheir sinensis with hepatopancreas symptoms were carried out by conventional paraffin section preparation method, and the enzymatic activities of the superoxide dismutase (SOD) , catalase (CAT), glutathione peroxidase(G-PX), malondialdehyde(MDA) in hepatopancreas were monitored by biochemical techniques. Results showed that the structure of hepatopancreas and gills changed seriously in diseased crabs. The pathological characteristics showed that the basic structure of hepatopancreas and gills were totally collapse, other organizations including heart, muscle also had varying degrees of pathological changes in the worst case. No obviously pathological change was found in intestinal. The three antioxidant enzyme activity in hepatopancreas and the content of malondialdehyde changed greatly. CAT activity in the hepatopancreas showed no significant change in slight hepatopancreas symptoms. With the deterioration of the hepatopancreas symptoms, the value of the three kinds of enzymes and MDA were showing significant differences.