| 荧光分光光度法测定大鼠不同脑区单胺类神经递质 |
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| 引用本文： | 张燕,王洪斌,杨海霞,陈清,赵丽. 荧光分光光度法测定大鼠不同脑区单胺类神经递质[J]. 东北农业大学学报, 2010, 41(1) |
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| 作者姓名： | 张燕 王洪斌 杨海霞 陈清 赵丽 |
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| 作者单位： | 东北农业大学动物医学学院,哈尔滨,150030;东北农业大学生命科学学院,哈尔滨,150030 |
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| 基金项目： | 国家自然科学基金项目(30671552);;黑龙江省自然科学基金重点项目(ZJN0503-01) |
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| 摘 要： | 介绍一种测定大鼠不同脑区单胺类神经递质的方法。采用荧光分光光度法同时测定大鼠不同脑区NE、DA和5-HT三种单胺类神经递质的含量。结果表明,荧光强度与浓度呈线性相关,NE、DA和5-HT的检测回收率分别为102.2%、95.4%和102.8%。该方法快速、灵敏、重复性较高,可为临床监测及科研提供参考。
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| 关 键 词： | 荧光分光光度法 不同脑区 单胺类神经递质 |
Content determination of monoamine neurotransmitter in different encephalic regions in rat by fluorospectrophotometry |
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| ZHANG Yan,WANG Hongbin,YANG Haixia,CHEN Qing,ZHAO Li. Content determination of monoamine neurotransmitter in different encephalic regions in rat by fluorospectrophotometry[J]. Journal of Northeast Agricultural University, 2010, 41(1) |
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| Authors: | ZHANG Yan WANG Hongbin YANG Haixia CHEN Qing ZHAO Li |
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| Affiliation: | 1.College of Veterinary Medicine;Northeast Agricultural University;Harbin 150030;China;2.College of Life Sciences;China |
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| Abstract: | The paper introduced a method for the determination of the monoamine neurotrans-mitter in different encephalic regions in rat.The contents of three monoamine neurotransmitter NE,L-DA and 5-HT in different encephalic regions in rat were measured by fluorospectrophotometry.The results showed that a linear dependence was presented between the fluorescence intensity and the concentration,the recovery rates of the NE,DA and 5-HT were 102.2%,95.4% and 102.8%,respectively.Thus,this method is rapid,sensitive and hi... |
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| Keywords: | fluorospectrophotometry different encephalic regions monoamine neurotransmitter |
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