龙麦19 Gli-A1/Glu-A3位点近等基因系低分子麦谷蛋白亚基(LGW-GS)鉴定 |
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引用本文: | 宋维富,赵海滨,张延滨,李集临,姜焕焕,丁琦,宋庆杰,于海洋,张春利,辛文利,肖志敏.龙麦19 Gli-A1/Glu-A3位点近等基因系低分子麦谷蛋白亚基(LGW-GS)鉴定[J].黑龙江农业科学,2012(2):1-5. |
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作者姓名: | 宋维富 赵海滨 张延滨 李集临 姜焕焕 丁琦 宋庆杰 于海洋 张春利 辛文利 肖志敏 |
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作者单位: | 哈尔滨师范大学生命科学与技术学院;黑龙江省农业科学院作物育种研究所 |
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基金项目: | 国家自然科学基金资助项目(30871525) |
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摘 要: | 为了鉴定小麦品种龙麦19(L-19,2*,7+9,2+12)和一对L-19的Gli-A1位点醇溶蛋白近等基因系L-19-626(2*,7+9,5+10)与L-19-613(2*,7+9,5+10)中与Gli-A1位点紧密连锁的Glu-A3位点低分子麦谷蛋白亚基(LMW-GS),利用一套Glu-A3位点特异低分子麦谷蛋白亚基STS标记对L-19、L-19-613、L-19-626及L-19/L-19-626的F2个体进行PCR扩增。结果表明:在具有醇溶蛋白Gli-A1m的个体中(L-19-626类型),Glu-A3e反应均为阳性,在不具有醇溶蛋白Gli-A1m的个体中(L-19和L-19-613类型),Glu-A3c反应均为阳性。说明编码L-19-626醇溶蛋白的Gli-A1 m基因与编码LMW-GS的Glu-A3e基因紧密连锁,编码L-19和L-19-613的Gli-A1位点醇溶蛋白基因(未命名)与编码LMW-GS的Glu-A3c基因紧密连锁。因此,L-19-626与L-19-613近等基因系品质间的差别很可能是由其LMW-GS Glu-A3e与Glu-A3c基因间的差异引起的。
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关 键 词: | 低分子麦谷蛋白亚基 近等基因系 STS标记 |
LMW-GS Identification at Gli-A1/GluA3 Loci in NILs of Wheat Variety Longmai 19 |
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Institution: | SONG Wei-fu1,2,ZHAO Hai-bin1,2,ZHANG Yan-bin1,2,LI Ji-lin1,JIANG Huan-huan1,2,DING Qi,SONG Qing-jie2(1.Life Science and Technology College of Harbin Normal University,Harbin,Heilongjiang 150025;2.Crop Breeding Institute of Heilongjiang Academy of Agricultural Sciences,Harbin,Heilongjiang 150086) |
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Abstract: | In order to identify LMW-GS at Glu-A3 locus linked with Gli-A1 locus in common wheat variety Longmai 19(L-19,HMW-GS 2*,7+9,2+12)and a pair of NILs(L-19-613 and L-19-626)at Gli-A1 locus with genetic background of Longmai 19 with HMW-GS 5+10,the materials that L-19,L-19-626 and L-19-613,F2 progeny of L-19/L-19-626 were assayed by a set STS markers of LMW-GS at Glu-A3 locus.The results showed that Glu-A3e amplified specific band in L-19-626 type materials with gliadin Gli-A1m.in L-19 and L-19-613 type materials without gliadin Gli-A1m,Glu-A3c amplified specific band.These results indicated the Gli-A1m at Gli-A1 locus was linked with LGW-GS Glu-A3e at Glu-A3 locus and the undesignated gliadin at Gli-A1 locus in L-19-613 and L-19 were linked with LGW-GS Glu-A3c at Glu-A3 locus.It was possible that quality difference between NILs L-19-626 and L-19-613 was due to LMW-GS gene difference of Glu-A3e and Glu-A3c at Glu-A3 locus. |
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Keywords: | LMW-GS NILs STS marker |
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