乳果糖对围产期犊牛免疫功能的影响

为研究乳果糖的替抗生素作用，选用同一牛场同一批从澳大利亚进口的青年荷斯坦奶牛所产公犊牛25头，按时间顺序分为5组，以随机单位组设计方法确定每组犊牛围产期内(初生至15日龄)乳料中不同的添加物：对照组（A）；金霉素组(B)，1万IU/头?d(3~15日龄)；金霉素+乳果糖组(C)，金霉素添加同B组、乳果糖添加同D组；乳果糖组(D)，10g/头?d；乳果糖组(E)，15g/头?d。实验结果显示，乳果糖对犊牛免疫器官指数，血液中T、B淋巴细胞百分含量无显著影响。淋巴细胞转化率从24 h开始，D、E组均与A组和B组组间差异极显著(p<0.01)，乳果糖与金霉素在促进淋巴细胞转化率方面存在协同效应。乳果糖能提高犊牛血液中C反应蛋白(CRP)含量及IgG含量，金霉素与乳果糖之间存在协同效应，但金霉素的增加效果不如添加乳果糖增加的效果。

Influence of Lactulose of Immunity on Calves in Perinatal Period

In order to study the effect of lactulose as substitute for antibiotic, this experiment had been carried on 25 newborn Holstein male calves which had been born by same passel youth cows from Australia in the same dairy farm. Calves had been divided into 5 groups (5 calves each group)conform to the born time. Unit group design had been used to make the certain additive of each calf in perinatal period(from firstborn to 15th day). Feed additives were: A: control group;B: aureomycin group, 10000 IU/d from 3rd day to 15th day; C: admixture group, lactulose & aureomycin; D: lactulose group, 10g/d one calf; E: lactulose group, 15g/d one claf. The results were as follows: Lactulose had no significantly effect(p>0.05) to immunity organ index, B&T lymphocyte quantity in the blood. From 24h, the lymphocyte conversion rate of lactulose groups were significantly more than control group and aureomycin group(p<0.01). Lactulose and aureomycin have coordination effect to promote conversion rate. Lactulose enhanced the content of CRP&IgG in the blood of calves. Aureomycin has coordination effect with the lactulose, but the promoted effect of aureomycin was inferior to lactulose.