高效液相色谱法研究啶菌噁唑(SYP-Z048)在番茄幼苗中的内吸传导特性

为明确啶菌噁唑(SYP-Z048)在植物体内的吸收传导特性及其在植物根部的吸收方式,于番茄幼苗根部或叶部施药后,采用高效液相色谱法(HPLC)分别测定了啶菌噁唑在根、茎、叶中的积累量;向含啶菌噁唑的培养液中分别添加羰基氰-间-氯苯腙、葡萄糖或色氨酸,以及在不同温度和pH条件下,分别测定了番茄幼苗根部对啶菌噁唑的吸收量及地上部分药剂积累量的变化。结果表明:50、100和200 mg/L啶菌噁唑处理番茄幼苗根部2 h后,在根部和茎部检测到目标药剂的含量分别为78.8、208.0、283.7μg/(g FW)和0.2、6.9、10.3μg/(g FW);16 h后,100 mg/L以上浓度处理组药剂可到达植株叶部;于中部叶片施加≥500 mg/mL的啶菌噁唑后72~124 h内,虽然在茎中未检测到药剂,但在根和上、下部叶片中均可检测到,表明药剂可在叶间传导转运至整个植株。不同浓度的羰基氰-间-氯苯腙、葡萄糖和色氨酸,以及低温条件和pH值等因子对番茄幼苗根部吸收药剂量和地上部分药剂积累量的影响与对照相比均无显著差异。研究表明,啶菌噁唑在番茄植株体内具有明显的向顶传导性及一定的向基传导性,同时其内吸传导过程以被动吸收占主导。

Study on the systemic properties of SYP-Z048 in tomato seedling using high performance liquid chromatography

To elucidate the uptake and translocation ability of 3-[5-(4-chloro phenyl)-2,3-dimethyl-3- isoxzaolidinyl] pyridine-3(SYP-Z048) in tomato seedlings and absorption mechanism of SYP-Z048 by roots, the contents of SYP-Z048 accumulated in roots, stems and leaves were determined respectively, using high performance liquid chromatography (HPLC) after application of SYP-Z048 to roots and leaves of tomato seedlings; the contents of SYP-Z048 were also determined in roots after incubation in SYP-Z048 solution amended with carbonyl cyanide m-chlorophehylhydrazone (CCCP), tryptophan and glucose, and under different temperature or pH values. The results showed that SYP-Z048 could be detected in the leaves and stems after SYP-Z048 application at 50, 100 and 200 mg/L to roots for 2 h, the contents were 78.8, 208.0, 283.7 μg/(g FW)and <0.2, 6.9, 10.3 μg/(g FW) (the amount of SYP-Z048 per gram of plant tissue), respectively; SYP-Z048 could be detected in leaves of the plant after application for 16 h at ≥100 mg/L. Moreover, after application onto the middle leaves of the plant for 72-124 h, SYP-Z048 could be detected in many parts except stems of the plant when treated at ≥500 mg/L, which indicated SYP-Z048 had been transported in the plant. The absorption of SYP-Z048 by roots was neither affected by CCCP, tryptophan or glucose, nor by low temperature or different pH values, compared to that of the control. The results of present study suggested that SYP-Z048 has strong acropetal translocation and weak basipetal translocation ability in tomato seedling, and the absorption is dominantly passive.