Expression of feline angiotensin converting enzyme 2 and its interaction with SARS-CoV S1 protein |
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Authors: | Guo Hongyan Guo Aizhen Wang Chong Yan Bangfen Lu Haisong Chen Huanchun |
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Affiliation: | aNational Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China |
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Abstract: | Feline angiotensin converting enzyme 2 (fACE2) gene was amplified from domestic cat lung with RT-PCR, cloned and sequenced. The complete coding region is 2418 bp in length and is the closest to human ACE2 among known ACE2 homologs of non-primate animals. The N terminal fragment 19– 367 aa was expressed in Escherishia coli. Both Western blotting and ELISA demonstrated that fACE2 could react with SARS-CoV S1 protein as efficiently as ACE2 of Vero E6 cells did. |
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Keywords: | Feline angiotensin converting enzyme 2(fACE2) SARS-CoV Spike protein Receptor Cats |
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