神经内分泌因子对体外培养新生犊牛肝细胞胰高血糖素受体mRNA丰度的影响

为阐明神经内分泌因子胰岛素(In)、胰高血糖素(GLN)、瘦素(LEP)在奶牛肝糖代谢、脂代谢中的调控作用，应用实时荧光定量PCR ( real time fluorescent quantitative PCR)法观察了神经内分泌因子对体外培养新生犊牛肝细胞胰高血糖素受体（glucagon receptor，GLNR）mRNA丰度的影响。结果显示：随着培养液中In的升高，GLNR mRNA表达逐渐增加（P<0.01）；随着培养液中GLN浓度的升高，GLNR mRNA表达逐渐降低（P<0.01）；而随着培养液中LEP浓度的升高，GLNR mRNA的表达呈现先升高后降低的趋势（P<0.01）。表明：神经内分泌因子In、GLN、LEP直接调控奶牛肝脏GLNR mRNA的表达。

Effects of Neuroendocrine Factors on Expression of GLNR mRNA in Monolayer Primary Cultured Neonatal Calf Hepatocytes in Vitro

In order to investigate the regulation role of neuroendocrine factors Insulin(In),glucagon(GLN) and leptin(LEP) in hepatocyte glycometabolism and lipometabolism of dairy cows,effects of In,GLN and LEP on expression of neonatal calf glucagon receptor(GLNR)mRNA in neonatal calf hepatocytes cultured in vitro were observed by real-time quantitative PCR.In results,the production of GLNR mRNA increased(P<0.01) along with increase of levels of In;and when glucagon in the culture media increased gradually,the production of GLNR mRNA decreased(P<0.01).Along with increase of leptin in the medium, the production of GLNR increased firstly and then decreased(P<0.01).These results demonstrated that In,GLN and LEP in the medium can directly regulate the expression of GLNR mRNA in the neonatal calf hepatocytes in vitro.