| 大孔树脂AB-8纯化麦冬多糖工艺的研究 |
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| 引用本文： | 毛讯.大孔树脂AB-8纯化麦冬多糖工艺的研究[J].安徽农业科学,2010,38(14):7308-7308,7338. |
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| 作者姓名： | 毛讯 |
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| 作者单位： | 郑州大学基础医学院,河南郑州,450000;漯河医学高等专科学校,河南漯河,462000 |
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| 摘 要： | 目的]研究大孔树脂纯化分离麦冬多糖的优化工艺条件。方法]比较了不同pH值、流速对麦冬多糖的纯化效果。结果]确定大孔树脂纯化麦冬多糖的层析条件为：上柱溶液pH值为8,洗脱溶液pH值为8,流速为1.0ml/min,在此条件下麦冬多糖纯度可达到81.0%,回收率71.2%。结论]该优化工艺条件可以作为麦冬多糖的纯化方式进行推广。
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| 关 键 词： | 麦冬 多糖 大孔树脂 AB-8 |
Study on the Purification Technology of Polysaccharide from Ophiopogo japonicus by Macroporous Resin AB-8 |
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| MAO Xun.Study on the Purification Technology of Polysaccharide from Ophiopogo japonicus by Macroporous Resin AB-8[J].Journal of Anhui Agricultural Sciences,2010,38(14):7308-7308,7338. |
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| Authors: | MAO Xun |
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| Institution: | MAO Xun (Basic Medical College,Zhengzhou University,Zhengzhou,Henan 450000) |
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| Abstract: | Objective]The purification and separation conditions of polysaccharide from Ophiopogon japonicus by macroporous resin. Method] The purification effects of different pH and flow rate on polysaccharide from Ophiopogon japonicus were compared. Result]The optimum technological conditions were determined as follows:the column solution pH =8,flow velocity of 1. 0 ml/min,elution pH =8. The results showed the purity reached 81. 0% and the recovery was 71. 2%. Conclusion]The conditions can be used as the purific... |
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| Keywords: | AB-8 |
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