| 猪附红细胞体PCR检测方法的建立 |
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| 引用本文： | 王妍 张守发 刘思国 王春来. 猪附红细胞体PCR检测方法的建立[J]. 中国农业科学, 2005, 38(10): 2153-2156 |
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| 作者姓名： | 王妍 张守发 刘思国 王春来 |
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| 作者单位： | [1]延边大学农学院动物医学系,龙井133400 [2]中国农业科学院哈尔滨兽医研究所,哈尔滨150001 |
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| 基金项目： | 吉林省科技厅项目（20020225）. |
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| 摘 要： | 根据已发表的部分猪附红细胞体序列设计了一对特异性引物，通过聚合酶链式反应（PCR）扩增出了一条约936 bp的基因片段，并成功地将该基因克隆于克隆载体PMD18-T，经酶切分析和PCR进一步鉴定后进行序列测定，结果表明，所克隆的目的基因与GenBank中发表的序列一致。并利用特异性引物初步建立了猪附红细胞体病PCR检测技术。
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| 关 键 词： | 猪 附红细胞体 克隆 PCR |
| 收稿时间： | 2004-08-23 |
| 修稿时间： | 2004-08-23 |
Establishment of the PCR Method for Detection of Suis Eperythrozoonsis |
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| WANG Yan, ZHANG Shou-fa, LIU Si-guo, WANG Chun-lai. Establishment of the PCR Method for Detection of Suis Eperythrozoonsis[J]. Scientia Agricultura Sinica, 2005, 38(10): 2153-2156 |
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| Authors: | WANG Yan ZHANG Shou-fa LIU Si-guo WANG Chun-lai |
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| Affiliation: | 1. Veterinary Medicine Department, Agricultural College, Yanbian University, Longjing 133400; 2.Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001 |
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| Abstract: | The 16s ribosomal RNA gene of suis eperythrozoon was amplified using gene specific primers from DNA that had been extracted from the blood of infected pig. The 936bp positive product was obtained by PCR and was cloned into the pMD18-T sector. The fragment was sequenced after restriction enzyme analysis and further defined with PCR. The sequence data demonstrated that the complete sequence was similar with the published. PCR assay for identification of eperythrozoonosis was developed. Results show that PCR assay has specificity. |
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| Keywords: | Swine Eperythrozoon Cloning PCR |
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