| 慢生型大豆根瘤菌nfe基因的分子克隆 |
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| 引用本文： | 周刚林,武波,唐东阶,柏学亮,马庆生.慢生型大豆根瘤菌nfe基因的分子克隆[J].广西农业生物科学,1999,18(1):29-32. |
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| 作者姓名： | 周刚林 武波 唐东阶 柏学亮 马庆生 |
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| 作者单位： | 1. 广西大学农学院
2. 广西大学农业分子遗传农业部重点开放实验室,南宁,530005 |
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| 基金项目： | 国家科技攻关项目;863-101-03-03-01; |
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| 摘 要： | ｎｆｅＣ基因是从慢生型大豆根瘤菌中克隆到的，与竞争结瘤有关的基因。本研究从慢生型大豆根瘤菌菌株ＧＸ２０１的ｐＬＡＦＲ３为载体的基因文库中，筛选出与ｎｆｅ同源基因克隆。以转座子Ｔｎ５ｇｕｓＡ５诱变获得了ｇｕｓ基因表达的Ｔｎ５ｇｕｓＡ５插入突变质粒。
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| 关 键 词： | 大豆根瘤菌 结瘤效率基因 分子克隆 基因文库 |
| 修稿时间： | 1998-12-17 |
Molecular Cloning of nfe Gene of Bradyrhizobium japonicum |
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| Zhou Ganglin,Wu Bo,Tang Dongjie,Bai Xueliang,Ma Qingsheng.Molecular Cloning of nfe Gene of Bradyrhizobium japonicum[J].Journal of Guangxi Agricultural and Biological Science,1999,18(1):29-32. |
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| Authors: | Zhou Ganglin Wu Bo Tang Dongjie Bai Xueliang Ma Qingsheng |
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| Abstract: | It had been shown that nfeC gene was essential for nodulation competitiveness of Bradyrhizobium japonicum.In this work, a genomic library of DNA of a B.japonicum strain GX201 was constructed in pLAFR3,a broad host range consmid vector within many Gramnegative hosts. The genomic libraries were screened for the clone showing structural homology to nfeC gene.The recombinant plasmids were constructed by using Tn5gusA5 transposon to mutagenize the clone. |
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| Keywords: | Bradyrhizobium japonicum nodule formation efficiency molecular clone genomic library |
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