枣基因组大小研究

采用流式细胞技术，以4个酸枣类型、9个枣品种的新鲜幼嫩叶片为材料，以Otto为解离液，日本晴（Oryza sativa ssp.japonica cv.Nipponbare）为外标，测定其基因组大小（2C值），比较枣和酸枣的2C值差异。结果表明，枣的基因组大小为(360.70±4.73)Mb，小于酸枣的基因组（388.20±8.32 Mb）；枣品种间基因组大小无显著差异，酸枣类型间差异显著，说明酸枣具有更高的基因组变异，且酸枣到枣的进化中可能伴随着基因组的缩减。

Comparative Analysis of Genome Sizes of Chinese Jujube

The genome sizes (2C-values) of 4 wild jujube species (Ziziphus jujuba var.spinosa) and 9 jujube varieties (Ziziphus jujuba) were tested by using flow cytometry technique.The fresh young leaves were used as materials,Otto (Oryza sativa L.ssp.japonica cv.Nipponbare) was used as the external reference.The genome size varieties were compared between jujube and wild jujube.The average genome size of jujube was estimated to be (360.70±4.73)Mb,which was smaller than the average genome size of wild jujube (388.20±8.32 Mb).There existed significant differences between the types of wild jujube in genome size but no significant differences between jujube varieties.The results indicated that the genome size variability of wild jujube was higher than jujube and the domestication may accompany with genome size reduction from wild jujube to jujube.