刻叶紫堇种子休眠特性及解除方法研究

从刻叶紫堇（Corydalis incisa）种子种皮透水性测定、种胚休眠特性检验、种子萌发抑制物的生物测定3个方面探讨了刻叶紫堇种子休眠的主要影响因素；通过不同层积方式和激素处理等方法探讨解除刻叶紫堇种子休眠的最佳处理方法。结果表明，种皮和种皮中含有的萌发抑制物并不是阻碍刻叶紫堇种子萌发的主要因素，而是由于刻叶紫堇种胚本身存在休眠，决定了其必须经过形态-生理后熟才能萌发；刻叶紫堇种子须经过沙藏层积才能打破休眠，本试验中低温转暖温沙藏是最佳层积方式，与GA₃浸种结合既可以提早打破休眠，还可以提高种子萌发率。

Study on Seed Dormancy Characteristics and Remove Methods of Corydalis incisa

This article discussed the main influencing factors of Corydalis incisa seed dormancy from three aspects,including seed skin permeability determination,embryo dormancy characteristic test,seed germination inhibition bioassay.Sand stratification and hormone treatment were adopted to lift the seed dormancy.The results showed that the seed skin and germination inhibitors in the skin were not the main influencing factors to block the seed germination,the seed embryo itself however,existed a dormancy behavior.Therefore,only the seeds passed through form-physiological after ripening,could seeds germination occur.C.incisa seeds required sand stratification to break dormancy,among them cold to warm temperature stratification was found to be the best stratification way,combined with GA₃ seed soaking,seed dormancy could be easily lifted while seed germination rate was improved.