24份杜鹃属植物的ISSR分子鉴定

采用ISSR分子标记对24份杜鹃属植物进行亲缘关系分析， 7个ISSR引物共扩增出92条谱带，其中89条为多态性谱带，多态性比率达96.7%。利用NTSYS-pc2.10e软件计算24份杜鹃属植物间的遗传相似系数，变化范围在0.456 5~0.782 6之间，平均相似系数为0.613 4。通过UPGMA聚类分析发现，24份杜鹃属植物被划分为3大类，聚类结果与地理分布存在一定的相关性，其中第2类与形态学分类基本吻合，表明24份杜鹃属植物的ISSR扩增图谱差异较大，易于区分，可用于杜鹃属植物的鉴定。

Identification of 24 Rhododendron Plants by ISSR Molecular Markers

ISSR molecular marker was used to study the genetic relationships of 24 samples of Rhododendron plants,a total of 92 bands were amplified by 7 ISSR primers,89 of which were polymorphic ones,accounting for 96.7% of the total.The genetic similarity coefficients varied from 0.456 5 to 0.782 6 with an average of 0.613 4,computed with software NTSYSpc2.10e.According to the UPGMA method,24 Rhododendron samples could be divided into three groups,clustering result with geographical distribution had certain relevance,and the second group was in accordance with the basic morphological classification.The result confirmed that 24 Rhododendron plants were easily identified owning to distinct diverse ISSR polymorphic bands,so this may be used for the identification of the Rhododendron plants.