SRAP标记分析陕西省主要茶树种质资源遗传多样性

用6份有代表性的陕西茶树资源材料，从154对SRAP引物组合中筛选出40 对扩增条带清晰、多态性高的引物组合，对陕西省50个茶树资源进行遗传多样性研究。结果表明，SRAP标记能够揭示材料间较高的遗传多样性，共检测到336个等位基因，每对引物组合可检测到5~13个等位位点，平均为8个；每个SRAP位点的遗传多态性信息含量在0.78~1.00之间，平均值为0.93。供试材料的遗传相似系数集中在0.73~0.95之间。可见，基于SRAP标记的陕西省主要茶树资源遗传多样性处于较高水平。

Analysis of SRAP Marker-based Polymorphism of the Main Tea Germplasms in Shaanxi

Genetic diversity of 50 tea germplasms in Shaanxi was investigated by using variable renaturing temperature-based PCR.Taking 6 representative tea plants as templates,40 pairs of SRAP markers were screened out from total 154 pairs of primers,which showed specific and obvious PCR products.The results demonstrated that total 336 alleles were verified using SRAP markers,and about 5-13 alleles were detected by each pair of molecular markers with an average of 8 alleles.The genetic polymorphism information contents of each SRAP locus were between 0.78-1.00,with an average of 0.93.Additionally,the genetic similarities of the 50 varieties were between 0.73-0.95.Based on SRAP markers,the genetic diversity of these tea materials was relatively high.