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| 84K杨树耐盐基因转化研究 | |
| 引用本文： | 樊军锋,韩一凡,李玲,彭学贤,李嘉瑞.84K杨树耐盐基因转化研究[J].西北林学院学报,2002,17(4):33-37. |
| 作者姓名： | 樊军锋 韩一凡 李玲 彭学贤 李嘉瑞 |
| 作者单位： | 1. 西北农林科技大学,林科院,陕西,杨陵,712100 2. 中国林业科学研究院,北京,100091 3. 中国科学院,微生物研究所,北京,100080 4. 西北农林科技大学,园艺学院,陕西,杨陵,712100 |
| 基金项目： | 陕西省科技厅科技攻关项目(2001K01-G7) |
| 摘 要： | 在已建立了84K杨叶片外植体再生系统的基础上，利用叶盘法首次开展了84K杨双价耐盐基因mtlD/gutD的转化研究，经诱导不定芽及诱导生根阶段卡那霉素（选择性抗生素）连续筛选，获得了16株卡那霉素抗性转化植株。抗性植株经PCR检测，有4株呈阳性。耐盐实验表明，3株阳性植株抗NaCl能力比对照有不同程度提高。PCR及耐盐实验初步证明双价耐盐基因转化获得成功。 |
| 关 键 词： | 基因转化 84K 耐盐基因 卡那霉素 叶盘法 |
| 文章编号： | 1001-7461(2002)04-0033-05 |
| 修稿时间： | 2002年5月27日 |
Salt-resistant Gene Transformation to Poplar 84K |
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| FAN Jun feng ,HAN Yi fan ,LI Ling ,PENG Xue xian ,LI Jia rui.Salt-resistant Gene Transformation to Poplar 84K[J].Journal of Northwest Forestry University,2002,17(4):33-37. | |
| Authors: | FAN Jun feng HAN Yi fan LI Ling PENG Xue xian LI Jia rui |
| Institution: | FAN Jun feng 1,HAN Yi fan 2,LI Ling 2,PENG Xue xian 3,LI Jia rui 1 |
| Abstract: | On the basis of establishment of leaf explant regeneration system of poplar 84K, transformation of mtlD/gutD gene to poplar 84k by means of leaf disc was for the first time undertaken. Through successive selection in the period of shoot and root induction under high lever kanamycin pressure, 16 kanamycin resistant regenerated plants was obtained, of which 4 was positive through PCR analysis. The salt resistant test of these 4 positive plants showed that 3 of them were enhanced in salt tolerance ability to some extent. PCR analysis and salt resistance test preliminarily indicated that the successful transformation had been made. |
| Keywords: | poplar 84K mtlD/gutD gene transformation salt resistance test |
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