野生松乳菇和野生红汁乳菇蛋白多糖的研究

 选用合适的溶剂、方法、条件从野生松乳菇和野生红汁乳菇中提取蛋白多糖。粗多糖经Sevage法脱游离蛋白、DEAE - 纤维素柱、SephadexG-100柱层析分离、纯化, 纯化的蛋白多糖进行紫外及红外光谱分析并测定蛋白质、多糖的含量及抗氧化能力。结果表明, 松乳菇蛋白多糖中蛋白质和多糖含量分别为24.53%和68.83%; 红汁乳菇中分别为15.53%和66.52% , 两蛋白多糖均具有体外抗氧化的能力。经鉴定松乳菇蛋白多糖中单糖组成主要是葡萄糖、果糖和甘露糖, 红汁乳菇蛋白多糖中单糖组成主要是葡萄糖、果糖和半乳糖。

Studies on the Proteoglycans Isolated from the Wild Lactarius deliciosus and Lactarius hatsudak

Proteoglycan was extracted from the Lactaricus deliciosus Gray and Lactarius hatsudak Tanake by appropriate solvent,method and condition. Proteoglycan was further isolated and purified by Sevage,DEAE-cellulose columns and SephdexG-100. After purified proteoglycans was analyzed by IR, UV-spectrum, the contents of protein and sugar were measured. The result shows that the protein and sugar content in the proteoglycan of Lactarius deliciosus were 24.53% and 68.83%, and that in proteoglycan of Lactarius hatsudak were 15.53% and 66.52%. At the same time two proteoglycans have antioxidation. TLC analysis of proteoglycan hydrolate showed that those sugars of Lactarius deliciosus were mainly composed of glucose,fructose and mannose while sugars of Lactarius hatsudak were mainly composed of glucose,fructose and xylose.