Identification of the hepatocyte growth factor receptor as the c-met proto-oncogene product. |
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| Authors: | D P Bottaro J S Rubin D L Faletto A M Chan T E Kmiecik G F Vande Woude S A Aaronson |
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| Affiliation: | Laboratory of Cellular and Molecular Biology, National Cancer Institute, Bethesda, MD 20892. |
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| Abstract: | Hepatocyte growth factor (HGF) is a plasminogen-like protein thought to be a humoral mediator of liver regeneration. A 145-kilodalton tyrosyl phosphoprotein observed in rapid response to HGF treatment of intact target cells was identified by immunoblot analysis as the beta subunit of the c-met proto-oncogene product, a membrane-spanning tyrosine kinase. Covalent cross-linking of 125I-labeled ligand to cellular proteins of appropriate size that were recognized by antibodies to c-met directly established the c-met product as the cell-surface receptor for HGF. |
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