Corticosteroid-induced alkaline phosphatase isoenzyme in the diagnosis of canine hypercorticism |
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Authors: | E Teske J Rothuizen J J de Bruijne A Rijnberk |
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Institution: | Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, State University of Utrecht, The Netherlands. |
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Abstract: | Corticosteroid-induced isoenzyme of alkaline phosphatase (AP) can easily be demonstrated in canine plasma as a routine procedure because of its greater heat stability at 65 degrees C in comparison with that of other AP-isoenzymes. In this study the accuracy of this test for the diagnosis of hypercorticism was investigated. The AP-65 degrees C test had its highest efficiency when applied to plasma AP levels exceeding 150 units/litre. In a group of 146 dogs, clinically suspected of having hyperadrenocorticism, the test had a sensitivity of 0.92 and a positive predictive value for a positive test result of 0.89. Its lack of specificity (0.44) makes it unsuitable as a diagnostic test. The main application of AP-65 degrees C is in detecting hypercorticism in dogs by routine laboratory measurements, as was demonstrated in 711 dogs, in which a positive predictive value for the presence of hypercorticism of 0.89 was found. |
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