| DNA荧光染色法和培养法在支原体检测中的比较 |
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| 引用本文： | 余华,杜永凤,严玉宝,何信群,胡娟,周岷江,崔鹏博. DNA荧光染色法和培养法在支原体检测中的比较[J]. 安徽农业科学, 2010, 38(8): 4039-4041 |
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| 作者姓名： | 余华 杜永凤 严玉宝 何信群 胡娟 周岷江 崔鹏博 |
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| 作者单位： | 1. 四川出入境检验检疫局,四川成都,610041
2. 中牧股份成都药械厂,四川成都,610066 |
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| 摘 要： | [目的]为检测支原体寻求一种有效、快速的方法。[方法]分别运用DNA荧光染色法和培养法对原材料、细胞和生物制品中的支原体污染进行检测,并对检测结果进行比较。[结果]DNA荧光染色法能够快速、准确地检测出细胞中支原体的污染;培养法在支原体检测中需要的时间较长,但能有效地对原材料、细胞和生物制品中支原体污染进行检测。[结论]有效地检测支原体应根据待检样品的特性来筛选最合适的方法。
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| 关 键 词： | DNA荧光染色法 培养法 支原体 检测 |
Comparison of the Method of DNA Fluorescent Staining and Cultivation Used for Detection of Mycoplasmas |
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| YU Hua et al. Comparison of the Method of DNA Fluorescent Staining and Cultivation Used for Detection of Mycoplasmas[J]. Journal of Anhui Agricultural Sciences, 2010, 38(8): 4039-4041 |
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| Authors: | YU Hua et al |
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| Affiliation: | Sichuan Entry-Exit Inspection and Quarantine Bureau of P.R.China;Chengdu;Sichuan 610041 |
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| Abstract: | [Objective] The aim of the study was to seek a good and effective method to detect the mycoplasma.[Method] The mycoplasma in starting material,cell,biological product were detected by DNA fluorescent staining and cultivation method.After experiment process and result were analyzed.[Result] The method of DNA fluorescent staining detecting mycoplasma in cell was feasible,sensitive and reliable.The method of Cultivation detecting mycoplasma in starting material,cell,biological product were feasible,sensitive a... |
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| Keywords: | DNA fluorescent Cultivation Mycoplasmas Detection |
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