肉果秤锤树叶提取物的抑菌作用研究

[目的]探讨秤锤树叶的逐级提取物的抑菌作用。[方法]采用试管稀释法测定秤锤树叶的各级提取物一总浸液、正丁醇萃取液、乙酸乙酯提取液对各试验菌的最小抑菌浓度(MIC)。[结果]各级提取物对试验菌分别有不同程度的抑菌作用,其中对金黄色葡萄球菌的抑菌作用最为明显;各提取物的抑菌强度依次为:乙酸乙酯萃取液(对4种实验菌均明显抑制:MIC≤31 mg/ml)、正丁醇萃取液(金黄色葡萄球菌:MIC≤62 mg/ml、其余3种菌的MIC相似:MIC≤125)、总浸液(仅对金黄色葡萄球菌抑制明显:MIC=125 mg/ml)。[结论]秤锤树叶的乙酸乙酯提取物具有广谱抗茵作用,其逐级提取物富集的抑茵活性逐级增强。

Study on the Bacteriostatic Action of the Extracts from the Leaves of Sinojackia sarcocarpa

[Objective] The research aimed to study the bacteriostatic action of the stepwise extracts from Sinojackia sarcocarpa.[Method] The minimal inhibitory concentration(MIC)of the stepwise extracts including total immersion liquid,n-butanol extract liquid and ethyl acetate extract liquid from S.sarcocarpa was determined by tube dilution method.[Result] The stepwise extracts had different bacteriostatic action on test bacteria.Its bacteriostatic action on Staphylococcus aureus was the most obvious.The bacteriostatic intensity of each extract was in order as follows: ethyl acetate extract liquid,n-butanol extract liquid and total immersion liquid.Ethyl acetate extract liquid all had the obvious inhibition with MIC ≤ 31 mg/m1.MIC of n-butanol extract liquid to S.aureus was equal to or less than 62 mg/ml and that to other three bacteria were equal to or less than 125 mg/ml.Total immersion liquid only had obvious inhibition to S.aureus.[Conclusion ] Ethyl acetate extract liquid from S.sarcocarpa had broad-spectrum bacteriostatic action and the accumulative inhibitory activity of its stepwise extracts was strengthened gradually.