| 互花米草中NHX基因片段的克隆与序列分析 |
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| 引用本文： | 赵祥强,汤薇,张燕,李鑫. 互花米草中NHX基因片段的克隆与序列分析[J]. 安徽农业科学, 2009, 37(36): 17878-17882 |
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| 作者姓名： | 赵祥强 汤薇 张燕 李鑫 |
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| 作者单位： | 南通大学生命科学学院,江苏南通,226007;南通大学生命科学学院,江苏南通,226007;南通大学生命科学学院,江苏南通,226007;南通大学生命科学学院,江苏南通,226007 |
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| 基金项目： | 江苏省高校自然科学基金项目，南通大学博士科研启动基金，江苏省植物功能基因组学重点实验室开放课题资助项目 |
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| 摘 要： | 利用同源克隆方法从盐生植物互花米草（Spartina alterniflora）中分离并克隆出Na~＋/H~＋逆向转运蛋白基因的1段cDNA片段,长度为951 bp,推测编码317个氨基酸。氨基酸序列的对位排列分析显示,该cDNA片段对应的氨基酸序列与已知的植物液泡膜型Na~＋/H~＋逆向转运蛋白的氨基酸序列有较高的相似性,且与禾本科植物中的同源物之间的关系更为密切。
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| 关 键 词： | 互花米草 耐盐性 同源克隆 NHX基因 |
Cloning and Sequence Analysis of A Fragment of NHX Exchanger Gene from Spartina alterniflora |
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| ZHAO Xiang-qiang et al. Cloning and Sequence Analysis of A Fragment of NHX Exchanger Gene from Spartina alterniflora[J]. Journal of Anhui Agricultural Sciences, 2009, 37(36): 17878-17882 |
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| Authors: | ZHAO Xiang-qiang et al |
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| Affiliation: | ZHAO Xiang-qiang et al(School of Life Sciences,Nantong University,Nantong,Jiangsu 226007) |
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| Abstract: | A cDNA fragment of Na~+/H~+ exchanger gene was isolated from a halophyte Spartina alterniflora by using the method of homologous gene cloning.This fragment consists of 951 bp,which can be deduced to encode 317 amino acids.According to the sequence analysis, it was found that the predicted amino acid sequence had high similarity to the vacuolar Na~+ /H~+ exchanger of other plants and tightly clustered with the homologies in cereals. |
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| Keywords: | Spartina alterniflora Salt tolerance Homologous gene cloning NHX gene |
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