| Construction and Characterization of Grass Carp （Ctenopharyngodon idellus） Fosmid Library |
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| 引用本文： | JIA Zhen-hu,LIN Chang-you,YANG Tian-yao,JIANG Yi-nan,XIA Chun. Construction and Characterization of Grass Carp （Ctenopharyngodon idellus） Fosmid Library[J]. 中国农业科学(英文版), 2010, 9(9): 1347-1352. DOI: 10.1016/S1671-2927(09)60225-X |
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| 作者姓名： | JIA Zhen-hu LIN Chang-you YANG Tian-yao JIANG Yi-nan XIA Chun |
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| 作者单位： | JIA Zhen-hu(Department of Microbiology and Immunology,College of Veterinary Medicine,China Agricultural University,Beijing 100193,P.R.China;College of Life Science,Shanxi Normal University,Linfen 041004,P.R.China);LIN Chang-you,YANG Tian-yao,JIANG Yi-nan,XIA Chun(Department of Microbiology and Immunology,College of Veterinary Medicine,China Agricultural University,Beijing 100193,P.R.China) |
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| 基金项目： | Acknowledgements The study was supported by the National Natural Science Foundation of China （30371 098）. |
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| 摘 要： | Grass carp （Ctenopharyngodon idellus） genomic fosmid library cotaining 129 014 clones was constructed and characterized from one diploid grass carp. The average insert size of the fosmid library was determined to be 35 kb by pulsed field gel electrophoresis, which is 4.1-fold coverage of the grass carp genome. To demonstrate the probability of picking the functional genes from the library, eleven functional genes were screened by three-dimensional PCR technique. The number of positive clones of these genes was from 1 to 6. So, this library may screen any useful genes from grass carp. This grass carp genome fosmid library will be integrated in the presently ongoing efforts to determine the sequence of the grass carp genome.
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| 关 键 词： | 草鱼 图书馆 基因组序列 脉冲场凝胶电泳 表征 功能基因 阳性克隆 PCR技术 |
| 收稿时间： | 2010-01-12 |
Construction and Characterization of Grass Carp(Ctenopharyngodon idellus)Fosmid Library |
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| JIA Zhen-hu,LIN Chang-you,YANG Tian-yao,JIANG Yi-nan,XIA Chun. Construction and Characterization of Grass Carp(Ctenopharyngodon idellus)Fosmid Library[J]. 《Agricultural Sciences in China》, 2010, 9(9): 1347-1352. DOI: 10.1016/S1671-2927(09)60225-X |
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| Authors: | JIA Zhen-hu LIN Chang-you YANG Tian-yao JIANG Yi-nan XIA Chun |
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| Affiliation: | 1. Department of Microbiology and Immunology,College of Veterinary Medicine,China Agricultural University,Beijing 100193,P.R.China;College of Life Science,Shanxi Normal University,Linfen 041004,P.R.China
2. Department of Microbiology and Immunology,College of Veterinary Medicine,China Agricultural University,Beijing 100193,P.R.China |
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| Abstract: | Grass carp(Ctenopharyngodon idellus)genomic fosmid library cotaining 129014 clones was constructed and characterized from one diploid grass carp.The average insert size of the fosmid library was determined to be 35 kb by pulsed field gel electrophoresis,which is 4.1-fold coverage of the grass carp genome.To demonstrate the probability of picking the functional genes from the library,eleven functional genes were screened by three-dimensional PCR technique.The number of positive clones of these genes was from 1 to 6.So,this library may screen any useful genes from grass carp.This grass carp genome fosmid library will be integrated in the presently ongoing efforts to determine the sequence of the grass carp genome. |
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| Keywords: | grass carp genome fosrnid library three-dimensional PCR |
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