The use of bulked segregant analysis to accumulate RAPD markers near a locus for beet cyst nematode resistance in Beta vulgaris |
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| Authors: | C Halldén T Säll K Olsson N-O Nilsson A Hjerdin |
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| Institution: | Hilleshög AB, Box 302, S-261 23 Landskrona, Sweden;Department of Genetics, Lund University, Sölvegatan 29, S-22362 Lund, Sweden;Department of Genetics, UmeåUniversity, S-901 87 Umeå, Sweden |
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| Abstract: | Bulked segregant analysis (BSA) was used to accumulate RAPD markers near the beet cyst nematode resistance locus Hslpro-1 of sugar beet (Beta vulgaris L.). Graphical genotypes constructed from RFLP data were utilized to select F2 individuals in (1) the construction of pools of plants used in the initial screening for polymorphisms, and (2) the selection of individual plants used to confirm the potential linkage. The pooled DNA samples were screened for polymorphisms using 668 RAPD primers. Forty-four candidate markers potentially linked to the region were analysed further using 14 segregating individuals. Close linkage was confirmed for 17 of the markers. Four of the RAPD markers were assigned map coordinates within the RFLP map. Three of these markers extended the RFLP map by 3cM. Altogether, the 8cM target interval contains 10 RFLP and 17 RAPD markers, corresponding to an average marker density of 0.3cM in the Hslpro-1 region. |
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| Keywords: | Beta vulgaris nematode resistance Hsl bulked segregant analysis RAPD graphical genotype |
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