聚酰胺-胺对大鼠腹腔巨噬细胞吞噬功能的影响

目的 研究聚酰胺-胺（poly-amidoamine，PAMAM）对大鼠腹腔巨噬细胞吞噬功能的影响。方法 采用CCK-8试剂盒检测PAMAM对巨噬细胞的细胞毒性。通过巨噬细胞吞噬印度墨汁实验和吞噬鸡红细胞实验，观察PAMAM对巨噬细胞吞噬功能的影响。采用扫描电子显微镜（SEM）观察巨噬细胞吞噬鸡红细胞的形貌。结果 0.1-10 mg/mL的G5 PAMAM-NH2和10 mg/mL的G4.5 PAMAM-COOH对巨噬细胞有毒性（P<0.05）。0.01 mg/mL的G5 PAMAM-NH2、G4.5 PAMAM-COOH、G5 PAMAM-OH对巨噬细胞均无毒性（P>0.05）。0.01 mg/mL的PAMAM对巨噬细胞吞噬鸡红细胞形貌、吞噬率和吞噬指数均无影响（P>0.05）。结论 PAMAM的浓度在0.01 mg/mL范围内，未观察到其对大鼠腹腔巨噬细胞吞噬功能有影响。

Effect of Poly(amidoamine) on the Phagocytosis of Peritoneal Macrophages in Rats

Objective To investigate the effect of poly(amidoamine), or PAMAM on the phagocytosis of peritoneal macrophage in rats. Methods The cytotoxicity of the PAMAM to the macrophage was detected by CCK-8 kit. The effect of PAMAM on the phagocytosis of the peritoneal macrophage was observed by the experiments of macrophages swallowing the India ink and chicken erythrocytes. The scanning electron microscope (SEM) was used to observe the morphology of the macrophage phagocytosis of chicken erythrocytes. Results There was toxicity to the macrophages with the concentrations of 0.1-10 mg/mL G5 PAMAM-NH2 and the concentration of 10 mg/mL G4.5 PAMAM-COOH (P<0.05). At the concentration of 0.01 mg/mL, all of the three kinds of the PAMAMs had no toxicity to the macrophages and had no effect on the morphology of the macrophage phagocytosis of chicken erythrocytes, the phagocytosis rate and index (P>0.05). Conclusion In the 0.01 mg/mL concentrations range, the PAMAMs have no effect on the phagocytosis of peritoneal macrophages in rats.