五种杀菌剂胁迫下灰葡萄孢产孢所需碳源种类分析

灰葡萄孢是引起作物灰霉病的病原菌,分生孢子是其传播的主要载体.本文采用代谢技术分析了灰葡萄孢对Biolog FF板碳源的利用及其产孢情况,并测定了在多菌灵、丙环唑、嘧霉胺、异菌脲和咪鲜胺5种杀菌剂胁迫下灰葡萄孢产孢所需碳源种类.结果表明:糖类、氨基酸类等92种碳源均能被灰葡萄孢代谢,其中,杏仁苷、L-阿拉伯糖等35种碳...

Carbon sources analysis for conidia formation of Botrytiscinerea under pressures of five fungicides

Botrytis cinerea is the pathogen of many crops gray mold disease and its conidia is the major carrier for disease epidemic. In this work, the carbon utilization and conidia formation of B. cinerea were analyzed by phenomic technology. In addition, the carbon sources for conidia production of this pathogen under five different fungicides (carbendazim, propiconazole, pyrimethanil, iprodione and prochloraz) were also tested. These results showed that about 92 carbon sources could be metabolized by B. cinerea, including carbohydrates, amino acids, and others. Among them, about 35 carbon sources such as amygdalin and L-arabinose could be metabolized to produce conidia. And about 57 carbon sources (tween 80, D-arabinose, glucuronic acid, etc.) could be metabolized by B. cinerea, but could not promote the conidia formation. When treated by 1 mg/L of carbendazim and 5 mg/L of prochloraz, and at both 1 and 10 mg/L of propiconazole, the carbon sources for conidia production of B. cinerea declined greatly. While treated for 0.08 and 1 mg/L of pyrimethanil or 0.1 and 5 mg/L of iprodione, they showed no inhibition. The results mentioned above revealed the carbon sources for conidia production of B. cinerea under chemical pressures of fungicides, which give us some constructive suggestions for the fungicide selection in gray mold management.