| 蜕壳周期内中华绒螯蟹钙含量、组织结构及相关基因表达变化 |
| |
| 引用本文： | 杨航,杨志刚,张龙. 蜕壳周期内中华绒螯蟹钙含量、组织结构及相关基因表达变化[J]. 水产科学, 2022, 41(1): 116-121. DOI: 10.16378/j.cnki.1003-1111.20100 |
| |
| 作者姓名： | 杨航 杨志刚 张龙 |
| |
| 作者单位： | 上海海洋大学,农业农村部淡水水产种质资源重点实验室,上海201306;上海海洋大学,农业农村部鱼类营养与环境生态研究中心,上海201306;上海海洋大学,水产科学国家级实验教学示范中心,上海201306 |
| |
| 基金项目： | 国家重点研发项目(2018YFD0900603);财政部和农业农村部现代农业产业技术体系专项资金资助项目(CARS-48);上海市科委工程技术中心能力提升项目(19DZ2284300). |
| |
| 摘 要： | 运用生化分析、组织切片和分子生物学等技术,测定蜕壳期(蜕下旧壳后0.5 h内)和蜕壳后期(蜕下旧壳0.5 h后至新壳未完全硬化之前)平均体质量(5.63±2.35)g中华绒螯蟹肝胰腺和鳃中的钙含量,观察肝胰腺和鳃组织结构变化,用荧光定量PCR技术分析肝胰腺和鳃中的钙网蛋白(CRT)基因的表达情况.试验结果表明,蜕壳后期...
|
| 关 键 词： | 中华绒螯蟹 蜕壳周期 钙含量 组织学 超微结构 钙网蛋白基因 |
Changes in Calcium Content,Histological Structure and Expression of Calreticulin Gene of Chinese Mitten Crab Eriocheir sinensis during Molting Cycle |
| |
| YANG Hang,YANG Zhigang,ZHANG Long. Changes in Calcium Content,Histological Structure and Expression of Calreticulin Gene of Chinese Mitten Crab Eriocheir sinensis during Molting Cycle[J]. Fisheries Science, 2022, 41(1): 116-121. DOI: 10.16378/j.cnki.1003-1111.20100 |
| |
| Authors: | YANG Hang YANG Zhigang ZHANG Long |
| |
| Affiliation: | (Key Laboratory of Freshwater Aquatic Genetic Resources,Ministry of Agriculture and Rural Affairs,Shanghai Ocean University,Shanghai 201306,China;Centre for Research on Environmental Ecology and Fish Nutrition(CREEFN),Ministry of Agriculture and Rural Affairs,Shanghai Ocean University,Shanghai 201306,China;National Demonstration Centre for Experimental Fisheries Science Education,Shanghai Ocean University,Shanghai 201306,China) |
| |
| Abstract: | In this study,calcium ion contents in hepatopancreas and gill of Chinese mitten crab Eriocheir sinensis with average body weight of(5.63±2.35)g were determined during molting cycle including the molting stage(within 0.5 h after the old shell molting)and the late molting stage(0.5 h after the old shell molting until the new shell was incompletely hardened)by biochemical analysis.The structure changes in hepatopancreas and gill were histologically observed during the molting cycle by paraffin section and HE staining,and the expression of calreticulin(CRT)gene was analyzed in hepatopancreas and gill by fluorescence quantitative PCR.The results showed that there was higher calcium content in hepatopancreas in the postmolt,while lower in gill during the ecdysis and postmolt.The histological observation showed that the cell contents in hepatopancreas were changed obviously during the molting cycle.In the gill,the cavity structure of marginal vessel was not recovered at the edge of the gill lamellae.The fluorescence quantitative analysis showed that the expression levels of calreticulin gene was decreased in hepatopancreas and gill from the intermolt to premolt(minimum)and increased from the premolt to postmolt.In the ecdysis,the minimal expression level was observed in the hepatopancreas and gill,which provided a theoretical basis for the study of molting and calcium transport of Chinese mitten crab. |
| |
| Keywords: | Eriocheir sinensis molting cycle calcium content histology ultrastructure calreticulin gene |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
