犊牛源雷氏普罗威登斯菌的分离鉴定及药物敏感性试验

目的] 确定内蒙古呼伦贝尔市新巴尔虎右旗家庭牧场犊牛腹泻的原因,并为该牧场犊牛腹泻的治疗提供药物选择方案。方法] 无菌采集发病犊牛肛拭子进行细菌培养鉴定及体外药物敏感性试验。结果] 从腹泻犊牛肛拭子样本分离到1株细菌,经培养后菌落形态一致,通过革兰染色鉴定、生理生化鉴定及16S rDNA鉴定确定该菌为雷氏普罗威登斯菌;该菌对氨曲南、头孢他啶、头孢噻肟、头孢吡肟、头孢曲松、四环素、氯霉素、呋喃妥因8种药物表现为耐药;对氨苄西林、庆大霉素2种药物表现为中度敏感;对头孢西丁、链霉素、哌拉西林、阿米卡星、氧氟沙星、诺氟沙星、左氧氟沙星7种药物表现为敏感。结论] 该养殖场的犊牛腹泻是由雷氏普罗威登斯菌所致,临床治疗过程中可使用链霉素。

Isolation,Identification and Antimicrobial Sensitivity Test of Providencia rettgeri from Calf

Objective] To determine the causive agent of calf diarrhea in a family livestock farm in New Barag Right Banner of Hulun Buir City, Inner Mongolia, and to provide references for drug selection for treatment of calf diarrhea in this farm. Method] Diseased calves′ anal swabs were obtained aseptically for bacterial culture, identification and in vitro antimicrobial sensitivity test. Result] A bacterial strain was isolated from the anal swab samples of the diarrhea calves. The colonies were similar in morphology in the culture. The strain was identified as Providencia rettgeri by Gram′s staining, physiological and biochemical identification, and 16S rDNA sequence analysis. It was resistant to aztreonam, ceftazidime, cefotaxime, cefepime, ceftriaxone, tetracycline, chloramphenicol, and furantoin, moderately sensitive to ampicillin and gentamicin, and sensitive to cefoxitin, streptomycin, piperacillin, amikacin, ofloxacin, norfloxacin, and levofloxacin. Conclusion] Providencia rettgeri is the causive agent of calf diarrhea in this farm. For clinical treatment, streptomycin is recommended.