| 家蚕病原性微孢子虫孢子表面蛋白的选择性分离与总蛋白比较分析 |
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| 引用本文： | 郭锡来 黄可威. 家蚕病原性微孢子虫孢子表面蛋白的选择性分离与总蛋白比较分析[J]. 蚕业科学, 1995, 21(4): 238-242 |
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| 作者姓名： | 郭锡来 黄可威 |
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| 作者单位： | 中国农业科学院蚕业研究所 |
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| 摘 要： | 选择性分离了Ｎｏｓｅｍａｂｏｍｂｙｃｉｓ孢子表面蛋白和总蛋白，采用ＳＤＳ－ＰＡＧＥ检测发现，孢子表面蛋白由分子量不同的３种亚基（３２０００、２６０００、２４５００Ｄａ）组成，其总蛋白至少包括２５个条带。同时将从养蚕生产中收集到的两种微粒子ＭＡ－１和ＭＤ－１以及从野外昆虫收集到的微孢子虫Ｐｈａ－Ｍ和Ｈａ－Ｍ与Ｎ．ｂｏｍｂｙｃｉｓ进行了比较，发现不同微孢子虫间蛋白组成有差异。
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| 关 键 词： | 家蚕，微孢子虫，孢子表面蛋白，孢子总蛋白 |
SELECTIVE SEPARATION OF EXOSPORAL PROTEIN AND COMPARATIVE ANALYSIS OF TOTAL SPORAL PROTEINS OF MICROSPORIDIAS PATHOGENIC TO SILKWORM BOMBYX MORI |
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| Guo Xijie Huang Kewei The Sericultural Research Institute,Chinese Academy of Agricultural Sciences,Zhenjiang ,China). SELECTIVE SEPARATION OF EXOSPORAL PROTEIN AND COMPARATIVE ANALYSIS OF TOTAL SPORAL PROTEINS OF MICROSPORIDIAS PATHOGENIC TO SILKWORM BOMBYX MORI[J]. Acta Sericologica Sinica, 1995, 21(4): 238-242 |
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| Authors: | Guo Xijie Huang Kewei The Sericultural Research Institute Chinese Academy of Agricultural Sciences Zhenjiang China) |
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| Affiliation: | Guo Xijie Huang Kewei The Sericultural Research Institute,Chinese Academy of Agricultural Sciences,Zhenjiang 212018,China) |
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| Abstract: | Exosporal and total proteins of the spores of Nosema bombycis were selectively separated and analysed with SDS-PAGE.The exosporal protein of N.bombycis consists of three subunits with the molecular weight of about 32 000,26 000 and 24 500 Daltons respectively.The SDS-PAGE profiles of total proteins of the N.bombycis spores contain at least 25 bands,At the same time,microsporidias MA-1 and MD-1,collected from commercial silkworm rearing,Pha-M and Ha-M,from mulberry insect pests Phyllobrotica armata and Hemerophila atrilineata respectively,were compared with N.bombycis on their exosporal and total proteins.Difference in protein components was found among those microsporidias. |
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| Keywords: | Bombyx mori Microsporidia Exosporal protei Total sporal protein |
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