3个杨树品种叶片再生体系的建立

以中林2001杨、南林95杨、南抗杨等3个杨树品种叶片为材料，选用15种诱导愈伤组织培养基、9种分化培养基和7种生根培养基，研究不同基因型、激素组合以及外植体状态对再生体系建立的影响。结果表明：3个品种的愈伤组织诱导、分化效果与NAA/6-BA比值有一定相关性，在愈伤组织诱导中NAA/6-BA比值在4:1~5:1之间，愈伤组织分化中NAA/6-BA比值大体在1:4~1:10之间；不同品种在上下表皮气孔数、诱导愈伤、分化再生培养上都存在一定的基因型差异；接种叶片的不同放置方式、叶片上的不同部分对愈伤组织诱导具有显著影响。

Establishment of Regeneration System from Leaves of 3 Populus Varieties

This paper deals with regeneration system from leaves of 3 Populus varieties (Populus deltoides cv.’zhonglin-2001’, Populus deltoids Bartr. cv. Lux × P . euramericana（Dode）Guineir cv. I-45 / 51, Populus deltoides cl. Nankang) with fifteen induced callus media, six differentiation media and seven rooting media. The effects of genotype, hormone concentration and explants condition were investigated. The results indicated that the effect on induced callus and differentiation of the three Populus varieties were related to the ratio of NAA to 6-BA. The ratio of NAA to 6-BA on induced callus was 4:1~5:1. The stoma number of leaves face and back, induced callus and plant regeneration of different varieties were different. There were significant effects on induced callus from leaves which were culture on medium with leaves face up or back up, leaves edge or middle.