| 枸杞nrDNA ITS测序鉴定的初步研究 |
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| 引用本文： | 石志刚,安巍,樊云芳,焦恩宁,赵建华,王亚军. 枸杞nrDNA ITS测序鉴定的初步研究[J]. 农业科学与技术, 2008, 9(1): 35-38 |
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| 作者姓名： | 石志刚 安巍 樊云芳 焦恩宁 赵建华 王亚军 |
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| 作者单位： | 宁夏枸杞工程技术研究中心,宁夏银川750002 |
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| 摘 要： | ITS区序列指DNA基因内的转录间隔区序列，包括ITSl、ITS2及5．8S3个区段。ITS区序列测定分析能够鉴别同属植物相似种以及药用植物混淆种。笔者以枸杞属内不同种间ITS序列为切入点，对枸杞nrDNA ITS序列进行分析研究，为从分子水平鉴定枸杞品种提供参考。
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| 关 键 词： | 枸杞 ITS区序列 DNA基因 测序鉴定 |
Preliminary Studies on Identification of Lycium Linn.Germplasm Resources by nrDNA ITS Sequencing |
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| SHI Zhi-gang,AN Wei,FAN Yun-fang,JIAO En-ning,ZHAO Jian-hua,WANG Ya-jun. Preliminary Studies on Identification of Lycium Linn.Germplasm Resources by nrDNA ITS Sequencing[J]. Agricultural Science ＆ Technology, 2008, 9(1): 35-38 |
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| Authors: | SHI Zhi-gang AN Wei FAN Yun-fang JIAO En-ning ZHAO Jian-hua WANG Ya-jun |
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| Affiliation: | (Ningxia Wolfberry Engineering and Technology Research Center, Yinehuan 750002) |
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| Abstract: | [Objective]The study aimed to identify wolfberry(Lycium Linn.) germplasm resources at molecular level by analyzing the nrDNA ITS sequence.[Method]Genomic DNA from wolfberry leaves extracted by modified CTAB method were regarded as templates for PCR amplification by specific primer,clone and sequencing.[Result]The nrDNA ITS sequences were obtained and then differentiated among three tested materials.[Conclusion]PCR amplification and sequencing on nrDNA ITS is a feasible approach to identify different wolfberry germplasm resources. |
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| Keywords: | Lycium L. ITS sequence DNA sequencing Identification |
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