| 连续多重PCR牛胚胎性别鉴定 |
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| 引用本文： | 吴阳升,刘明军,郭志勤.连续多重PCR牛胚胎性别鉴定[J].新疆农业大学学报,2004,27(2):29-32. |
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| 作者姓名： | 吴阳升 刘明军 郭志勤 |
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| 作者单位： | 1. 新疆畜牧科学院,农业部家畜繁育重点开放实验室,乌鲁木齐,830000;新疆农业大学,农学院,乌鲁木齐,830052
2. 新疆畜牧科学院,农业部家畜繁育重点开放实验室,乌鲁木齐,830000 |
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| 摘 要： | 本实验以BOV97M序列和牛的1．715卫星DNA序列设计引物进行PCR扩增，其中前者为雄性特异扩增，后者为雌雄共同扩增，以进行牛的性别鉴定。PCR扩增共进行43个循环，其中前11个循环为雄性特异扩增，在随后的33个循环中，再加入内标引物，即进行多重PCR扩增，其灵敏度可达到10pg级(约3个细胞)。
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| 关 键 词： | 性别鉴定 PCR 牛 |
| 文章编号： | 1007-8614(2004)02-0029-04 |
| 修稿时间： | 2004年4月7日 |
Sex determination of bovine embryo using consecutive and multiplex polymerase chain reaction (PCR) |
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| WU Yang-sheng.Sex determination of bovine embryo using consecutive and multiplex polymerase chain reaction (PCR)[J].Journal of Xinjiang Agricultural University,2004,27(2):29-32. |
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| Authors: | WU Yang-sheng |
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| Institution: | WU Yang-sheng~ |
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| Abstract: | The BOV97M and bovine 1.715 satelite sequences were selected to amplify male and bovine-specific DNA, respectively. In all 43 cycles were conducted, the first 11 PCR cycles were done with male-specific primer followed by an additional 33 cycles with bovine-specific primer namely Consecutive and Multiplex Polymerase Chain Reaction (PCR). This PCR method was applied successfully to use templates of 10pg (about 3 cells). |
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| Keywords: | sex determination polymerase chain reaction (PCR) bovine |
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